Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications

Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications

The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H2-based biofuel application as it favours H2 oxidation and is relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce and purify an active SH are described, based on...

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Main Authors: Jugder, Bat-Erdene, Lebhar, Helene, Aguey-Zinsou, Kondo-Francois, Marquis, Christopher P.
Format: Online
Language:English
Published: Elsevier 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816682/
id pubmed-4816682
recordtype oai_dc
spelling pubmed-48166822016-04-13 Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications Jugder, Bat-Erdene Lebhar, Helene Aguey-Zinsou, Kondo-Francois Marquis, Christopher P. Biochemistry, Genetics and Molecular Biology The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H2-based biofuel application as it favours H2 oxidation and is relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce and purify an active SH are described, based on the methods previously reported [1], [2], [3], [4]. Our modifications are: Elsevier 2016-03-22 /pmc/articles/PMC4816682/ /pubmed/27077052 http://dx.doi.org/10.1016/j.mex.2016.03.005 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Jugder, Bat-Erdene
Lebhar, Helene
Aguey-Zinsou, Kondo-Francois
Marquis, Christopher P.
spellingShingle Jugder, Bat-Erdene
Lebhar, Helene
Aguey-Zinsou, Kondo-Francois
Marquis, Christopher P.
Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications
author_facet Jugder, Bat-Erdene
Lebhar, Helene
Aguey-Zinsou, Kondo-Francois
Marquis, Christopher P.
author_sort Jugder, Bat-Erdene
title Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications
title_short Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications
title_full Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications
title_fullStr Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications
title_full_unstemmed Production and purification of a soluble hydrogenase from Ralstonia eutropha H16 for potential hydrogen fuel cell applications
title_sort production and purification of a soluble hydrogenase from ralstonia eutropha h16 for potential hydrogen fuel cell applications
description The soluble hydrogenase (SH) from Ralstonia eutropha H16 is a promising candidate enzyme for H2-based biofuel application as it favours H2 oxidation and is relatively oxygen-tolerant. In this report, bioprocess development studies undertaken to produce and purify an active SH are described, based on the methods previously reported [1], [2], [3], [4]. Our modifications are:
publisher Elsevier
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4816682/
_version_ 1613560697716736000

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