Nucleic acids delivery methods for genome editing in zygotes and embryos: the old, the new, and the old-new
In the recent years, sequence-specific nucleases such as ZFNs, TALENs, and CRISPR/Cas9 have revolutionzed the fields of animal genome editing and transgenesis. However, these new techniques require microinjection to deliver nucleic acids into embryos to generate gene-modified animals. Microinjection...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
BioMed Central
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4815204/ |