GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance

GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance

Gpr21 KO mice generated with Gpr21 KO ES cells obtained from Deltagen showed improved glucose tolerance and insulin sensitivity when fed a high fat diet. Further mRNA expression analysis revealed changes in Rabgap1 levels and raised the possibility that Rabgap1 gene may have been modified. To asses...

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Main Authors: Wang, Jinghong, Pan, Zheng, Baribault, Helene, Chui, Danny, Gundel, Caroline, Véniant, Murielle
Format: Online
Language:English
Published: F1000Research 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813635/
id pubmed-4813635
recordtype oai_dc
spelling pubmed-48136352016-04-13 GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance Wang, Jinghong Pan, Zheng Baribault, Helene Chui, Danny Gundel, Caroline Véniant, Murielle Research Note Gpr21 KO mice generated with Gpr21 KO ES cells obtained from Deltagen showed improved glucose tolerance and insulin sensitivity when fed a high fat diet. Further mRNA expression analysis revealed changes in Rabgap1 levels and raised the possibility that Rabgap1 gene may have been modified. To assess this hypothesis a new Gpr21 KO mouse line using TALENS technology was generated. Gpr21 gene deletion was confirmed by PCR and Gpr21 and Rabgap1 mRNA expression levels were determined by RT-PCR. The newly generated Gpr21 KO mice when fed a normal or high fat diet chow did not maintain their improved metabolic phenotype. In conclusion, Rabgap1 disturbance mRNA expression levels may have contributed to the phenotype of the originally designed Gpr21 KO mice. F1000Research 2016-06-17 /pmc/articles/PMC4813635/ /pubmed/27081476 http://dx.doi.org/10.12688/f1000research.7822.2 Text en Copyright: © 2016 Wang J et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Wang, Jinghong
Pan, Zheng
Baribault, Helene
Chui, Danny
Gundel, Caroline
Véniant, Murielle
spellingShingle Wang, Jinghong
Pan, Zheng
Baribault, Helene
Chui, Danny
Gundel, Caroline
Véniant, Murielle
GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
author_facet Wang, Jinghong
Pan, Zheng
Baribault, Helene
Chui, Danny
Gundel, Caroline
Véniant, Murielle
author_sort Wang, Jinghong
title GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
title_short GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
title_full GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
title_fullStr GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
title_full_unstemmed GPR21 KO mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
title_sort gpr21 ko mice demonstrate no resistance to high fat diet induced obesity or improved glucose tolerance
description Gpr21 KO mice generated with Gpr21 KO ES cells obtained from Deltagen showed improved glucose tolerance and insulin sensitivity when fed a high fat diet. Further mRNA expression analysis revealed changes in Rabgap1 levels and raised the possibility that Rabgap1 gene may have been modified. To assess this hypothesis a new Gpr21 KO mouse line using TALENS technology was generated. Gpr21 gene deletion was confirmed by PCR and Gpr21 and Rabgap1 mRNA expression levels were determined by RT-PCR. The newly generated Gpr21 KO mice when fed a normal or high fat diet chow did not maintain their improved metabolic phenotype. In conclusion, Rabgap1 disturbance mRNA expression levels may have contributed to the phenotype of the originally designed Gpr21 KO mice.
publisher F1000Research
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4813635/
_version_ 1613559721076195328

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