Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets

The intestinal upper villus epithelial cells represent the differentiated epithelial cells and play key role in digesting and absorbing lumenal nutrients. Weaning stress commonly results in a decrease in villus height and intestinal dysfunction in piglets. However, no study have been conducted to te...

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Main Authors:	Yang, Huansheng, Xiong, Xia, Wang, Xiaocheng, Tan, Bie, Li, Tiejun, Yin, Yulong
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811545/

id	pubmed-4811545
recordtype	oai_dc
spelling	pubmed-48115452016-04-05 Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets Yang, Huansheng Xiong, Xia Wang, Xiaocheng Tan, Bie Li, Tiejun Yin, Yulong Research Article The intestinal upper villus epithelial cells represent the differentiated epithelial cells and play key role in digesting and absorbing lumenal nutrients. Weaning stress commonly results in a decrease in villus height and intestinal dysfunction in piglets. However, no study have been conducted to test the effects of weaning on the physiology and functions of upper villus epithelial cells. A total of 40 piglets from 8 litters were weaned at 14 days of age and one piglet from each litter was killed at 0 d (w0d), 1 d (w1d), 3 d (w3d), 5 d (w5d), and 7 d (w7d) after weaning, respectively. The upper villus epithelial cells in mid-jejunum were isolated using the distended intestinal sac method. The expression of proteins in upper villus epithelial cells was analyzed using the isobaric tags for relative and absolute quantification or Western blotting. The expression of proteins involved in energy metabolism, Golgi vesicle transport, protein amino acid glycosylation, secretion by cell, transmembrane transport, ion transport, nucleotide catabolic process, translational initiation, and epithelial cell differentiation and apoptosis, was mainly reduced during the post-weaning period, and these processes may be regulated by mTOR signaling pathway. These results indicated that weaning inhibited various cellular processes in jejunal upper villus epithelial cells, and provided potential new directions for exploring the effects of weaning on the functions of intestine and improving intestinal functions in weaning piglets. Public Library of Science 2016-03-29 /pmc/articles/PMC4811545/ /pubmed/27022727 http://dx.doi.org/10.1371/journal.pone.0150216 Text en © 2016 Yang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yang, Huansheng Xiong, Xia Wang, Xiaocheng Tan, Bie Li, Tiejun Yin, Yulong
spellingShingle	Yang, Huansheng Xiong, Xia Wang, Xiaocheng Tan, Bie Li, Tiejun Yin, Yulong Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets
author_facet	Yang, Huansheng Xiong, Xia Wang, Xiaocheng Tan, Bie Li, Tiejun Yin, Yulong
author_sort	Yang, Huansheng
title	Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets
title_short	Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets
title_full	Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets
title_fullStr	Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets
title_full_unstemmed	Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets
title_sort	effects of weaning on intestinal upper villus epithelial cells of piglets
description	The intestinal upper villus epithelial cells represent the differentiated epithelial cells and play key role in digesting and absorbing lumenal nutrients. Weaning stress commonly results in a decrease in villus height and intestinal dysfunction in piglets. However, no study have been conducted to test the effects of weaning on the physiology and functions of upper villus epithelial cells. A total of 40 piglets from 8 litters were weaned at 14 days of age and one piglet from each litter was killed at 0 d (w0d), 1 d (w1d), 3 d (w3d), 5 d (w5d), and 7 d (w7d) after weaning, respectively. The upper villus epithelial cells in mid-jejunum were isolated using the distended intestinal sac method. The expression of proteins in upper villus epithelial cells was analyzed using the isobaric tags for relative and absolute quantification or Western blotting. The expression of proteins involved in energy metabolism, Golgi vesicle transport, protein amino acid glycosylation, secretion by cell, transmembrane transport, ion transport, nucleotide catabolic process, translational initiation, and epithelial cell differentiation and apoptosis, was mainly reduced during the post-weaning period, and these processes may be regulated by mTOR signaling pathway. These results indicated that weaning inhibited various cellular processes in jejunal upper villus epithelial cells, and provided potential new directions for exploring the effects of weaning on the functions of intestine and improving intestinal functions in weaning piglets.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4811545/
_version_	1613559063786815488

Effects of Weaning on Intestinal Upper Villus Epithelial Cells of Piglets

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