Transcriptome Analysis of Cultured Limbal Epithelial Cells on an Intact Amniotic Membrane following Hypothermic Storage in Optisol-GS

Transcriptome Analysis of Cultured Limbal Epithelial Cells on an Intact Amniotic Membrane following Hypothermic Storage in Optisol-GS

The aim of the present study was to investigate the molecular mechanisms underlying activation of cell death pathways using genome-wide transcriptional analysis in human limbal epithelial cell (HLEC) cultures following conventional hypothermic storage in Optisol-GS. Three-week HLEC cultures were sto...

Full description

Bibliographic Details
Main Authors:	Paaske Utheim, Tor, Salvanos, Panagiotis, Aass Utheim, Øygunn, Ræder, Sten, Pasovic, Lara, Olstad, Ole Kristoffer, Fideliz de la Paz, Maria, Sehic, Amer
Format:	Online
Language:	English
Published:	MDPI 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4810063/

Similar Items

Pre-Clinical Cell-Based Therapy for Limbal Stem Cell Deficiency
by: Sehic, Amer, et al.
Published: (2015)

Culture of Oral Mucosal Epithelial Cells for the Purpose of Treating Limbal Stem Cell Deficiency
by: Paaske Utheim, Tor, et al.
Published: (2016)

Serum-Free and Xenobiotic-Free Preservation of Cultured Human Limbal Epithelial Cells
by: Utheim, Oeygunn, et al.
Published: (2015)

The Three Paralogous MicroRNA Clusters in Development and Disease, miR-17-92, miR-106a-363, and miR-106b-25
by: Khuu, Cuong, et al.
Published: (2016)

Glutathione Preservation during Storage of Rat Lenses in Optisol-GS and Castor Oil
by: Holm, Thomas, et al.
Published: (2013)

Safety and Efficacy of Epithelium-On Corneal Collagen Cross-Linking Using a Multifactorial Approach to Achieve Proper Stromal Riboflavin Saturation
by: Stojanovic, Aleksandar, et al.
Published: (2012)

Keratometric Index Obtained by Fourier-Domain Optical Coherence Tomography
by: Hua, Yanjun, et al.
Published: (2015)

Storage Temperature Alters the Expression of Differentiation-Related Genes in Cultured Oral Keratinocytes
by: Utheim, Tor Paaske, et al.
Published: (2016)

Assessment of refractive astigmatism and simulated therapeutic refractive surgery strategies in coma-like-aberrations-dominant corneal optics
by: Zhou, Wen, et al.
Published: (2016)

Corneal Collagen Cross-Linking with and without Epithelial Removal: A Contralateral Study with 0.5% Hypotonic Riboflavin Solution
by: Stojanovic, Aleksandar, et al.
Published: (2014)

Cultured epidermal stem cells in regenerative medicine
by: Catherine J. Jackson, et al.
Published: (2017-07-01)

Concise Review: Comparison of Culture Membranes Used for Tissue Engineered Conjunctival Epithelial Equivalents
by: Eidet, Jon Roger, et al.
Published: (2015)

Corneal collagen cross-linking (CXL) in thin corneas
by: Chen, Xiangjun, et al.
Published: (2015)

Interdisciplinary, Comprehensive Oral and Ocular Evaluation of Patients with Primary Sjögren’s Syndrome
by: Behzod Tashbayev, et al.
Published: (2017-09-01)

Donor cornea transfer from Optisol GS to organ culture storage: a two-step procedure to increase donor tissue lifespan
by: Haug, Kristiane, et al.
Published: (2013)

Effect of Cryopreserved Amniotic Membrane Orientation on the Expression of Limbal Mesenchymal and Epithelial Stem Cell Markers in Prolonged Limbal Explant Cultures
by: Lužnik, Zala, et al.
Published: (2016)

Objective assessment of changes in nuclear morphology and cell distribution following induction of apoptosis
by: Eidet, Jon R, et al.
Published: (2014)

Effect of Storage Temperature on Key Functions of Cultured Retinal Pigment Epithelial Cells
by: Pasovic, Lara, et al.
Published: (2015)

Antioxidants Improve the Viability of Stored Adult Retinal Pigment Epithelial-19 Cultures
by: Pasovic, Lara, et al.
Published: (2014)

Dry Eye Disease Patients with Xerostomia Report Higher Symptom Load and Have Poorer Meibum Expressibility
by: Fostad, Ida G., et al.
Published: (2016)

Post-hypothermic cardiac left ventricular systolic dysfunction after rewarming in an intact pig model
by: Filseth, Ole Magnus, et al.
Published: (2010)

One-Step Transepithelial Topography-Guided Ablation in the Treatment of Myopic Astigmatism
by: Stojanovic, Aleksandar, et al.
Published: (2013)

Evaluation of Equivalent Keratometry Readings Obtained by Pentacam HR (High Resolution)
by: Hua, Yanjun, et al.
Published: (2016)

Reliability of Corneal Dynamic Scheimpflug Analyser Measurements in Virgin and Post-PRK Eyes
by: Chen, Xiangjun, et al.
Published: (2014)

Optimization of Storage Temperature for Cultured ARPE-19 Cells
by: Pasovic, Lara, et al.
Published: (2013)

Fetal extraction in intact amniotic sac in twins
by: N R Akhmadeyev, et al.
Published: (2012-12-01)

Comparison of upstream regulators in human ex vivo cultured cornea limbal epithelial stem cells and differentiated corneal epithelial cells
by: Veréb, Zoltán, et al.
Published: (2013)

The Limbal Epithelial Progenitors in the Limbal Niche Environment
by: Zhang, Yuan, et al.
Published: (2016)

Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium
by: Navaratnam, Jesintha, et al.
Published: (2015)

The Silk-protein Sericin Induces Rapid Melanization of Cultured Primary Human Retinal Pigment Epithelial Cells by Activating the NF-κB Pathway
by: Eidet, J. R., et al.
Published: (2016)

Glutaraldehyde cross-linking of amniotic membranes affects their nanofibrous structures and limbal epithelial cell culture characteristics
by: Lai, Jui-Yang, et al.
Published: (2013)

Effects of amniotic membrane transplantation combined with limbal stem cell autograft transplantation on ocular burns
by: Guo-Ning Ye, et al.
Published: (2018-10-01)

Cultivation and Characterization of Cornea Limbal Epithelial Stem Cells on Lens Capsule in Animal Material-Free Medium
by: Albert, Réka, et al.
Published: (2012)

Limbal Stem Cells in Review
by: Ebrahimi, Marzieh, et al.
Published: (2009)

Hypothermic overdose, not all bad?
by: Petterson, Timothy, et al.
Published: (2013)

A comparison of stem cell-related gene expression in the progenitor-rich limbal epithelium and the differentiating central corneal epithelium
by: Nieto-Miguel, Teresa, et al.
Published: (2011)

Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity
Published: (1992)

Management of pediatric corneal limbal dermoids
by: Pirouzian, Amir
Published: (2013)

Osborn waves in a hypothermic patient
by: Serafi, Sami W., et al.
Published: (2012)

Stabilization of collagen nanofibers with l-lysine improves the ability of carbodiimide cross-linked amniotic membranes to preserve limbal epithelial progenitor cells
by: Lai, Jui-Yang, et al.
Published: (2014)