Effects of Disinfectants on Larval Development of Ascaris suum Eggs

The objective of this study was to evaluate the effects of several different commercial disinfectants on the embryogenic development of Ascaris suum eggs. A 1-ml aliquot of each disinfectant was mixed with approximately 40,000 decorticated or intact A. suum eggs in sterile tubes. After each treatmen...

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Main Authors:	Oh, Ki-Seok, Kim, Geon-Tae, Ahn, Kyu-Sung, Shin, Sung-Shik
Format:	Online
Language:	English
Published:	The Korean Society for Parasitology and Tropical Medicine 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792319/

id	pubmed-4792319
recordtype	oai_dc
spelling	pubmed-47923192016-03-17 Effects of Disinfectants on Larval Development of Ascaris suum Eggs Oh, Ki-Seok Kim, Geon-Tae Ahn, Kyu-Sung Shin, Sung-Shik Brief Communication The objective of this study was to evaluate the effects of several different commercial disinfectants on the embryogenic development of Ascaris suum eggs. A 1-ml aliquot of each disinfectant was mixed with approximately 40,000 decorticated or intact A. suum eggs in sterile tubes. After each treatment time (at 0.5, 1, 5, 10, 30, and 60 min), disinfectants were washed away, and egg suspensions were incubated at 25˚C in distilled water for development of larvae inside. At 3 weeks of incubation after exposure, ethanol, methanol, and chlorohexidin treatments did not affect the larval development of A. suum eggs, regardless of their concentration and treatment time. Among disinfectants tested in this study, 3% cresol, 0.2% sodium hypochlorite and 0.02% sodium hypochlorite delayed but not inactivated the embryonation of decorticated eggs at 3 weeks of incubation, because at 6 weeks of incubation, undeveloped eggs completed embryonation regardless of exposure time, except for 10% povidone iodine. When the albumin layer of A. suum eggs remained intact, however, even the 10% povidone iodine solution took at least 5 min to reasonably inactivate most eggs, but never completely kill them with even 60 min of exposure. This study demonstrated that the treatment of A. suum eggs with many commercially available disinfectants does not affect the embryonation. Although some disinfectants may delay or stop the embryonation of A. suum eggs, they can hardly kill them completely. The Korean Society for Parasitology and Tropical Medicine 2016-02 2016-02-26 /pmc/articles/PMC4792319/ /pubmed/26951988 http://dx.doi.org/10.3347/kjp.2016.54.1.103 Text en © 2016, Korean Society for Parasitology and Tropical Medicine This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Oh, Ki-Seok Kim, Geon-Tae Ahn, Kyu-Sung Shin, Sung-Shik
spellingShingle	Oh, Ki-Seok Kim, Geon-Tae Ahn, Kyu-Sung Shin, Sung-Shik Effects of Disinfectants on Larval Development of Ascaris suum Eggs
author_facet	Oh, Ki-Seok Kim, Geon-Tae Ahn, Kyu-Sung Shin, Sung-Shik
author_sort	Oh, Ki-Seok
title	Effects of Disinfectants on Larval Development of Ascaris suum Eggs
title_short	Effects of Disinfectants on Larval Development of Ascaris suum Eggs
title_full	Effects of Disinfectants on Larval Development of Ascaris suum Eggs
title_fullStr	Effects of Disinfectants on Larval Development of Ascaris suum Eggs
title_full_unstemmed	Effects of Disinfectants on Larval Development of Ascaris suum Eggs
title_sort	effects of disinfectants on larval development of ascaris suum eggs
description	The objective of this study was to evaluate the effects of several different commercial disinfectants on the embryogenic development of Ascaris suum eggs. A 1-ml aliquot of each disinfectant was mixed with approximately 40,000 decorticated or intact A. suum eggs in sterile tubes. After each treatment time (at 0.5, 1, 5, 10, 30, and 60 min), disinfectants were washed away, and egg suspensions were incubated at 25˚C in distilled water for development of larvae inside. At 3 weeks of incubation after exposure, ethanol, methanol, and chlorohexidin treatments did not affect the larval development of A. suum eggs, regardless of their concentration and treatment time. Among disinfectants tested in this study, 3% cresol, 0.2% sodium hypochlorite and 0.02% sodium hypochlorite delayed but not inactivated the embryonation of decorticated eggs at 3 weeks of incubation, because at 6 weeks of incubation, undeveloped eggs completed embryonation regardless of exposure time, except for 10% povidone iodine. When the albumin layer of A. suum eggs remained intact, however, even the 10% povidone iodine solution took at least 5 min to reasonably inactivate most eggs, but never completely kill them with even 60 min of exposure. This study demonstrated that the treatment of A. suum eggs with many commercially available disinfectants does not affect the embryonation. Although some disinfectants may delay or stop the embryonation of A. suum eggs, they can hardly kill them completely.
publisher	The Korean Society for Parasitology and Tropical Medicine
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4792319/
_version_	1613552013542424576

Effects of Disinfectants on Larval Development of Ascaris suum Eggs

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