Gorab is required for dermal condensate cells to respond to hedgehog signals during hair follicle morphogenesis
GORAB is a golgin that localizes predominantly at the Golgi apparatus and physically interacts with small GTPases. GORAB is ubiquitously expressed in mammalian tissues, including the skin. However, the biological function of this golgin in skin is unknown. Here, we report that disrupting the express...
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2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4789774/ |
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pubmed-47897742016-05-24 Gorab is required for dermal condensate cells to respond to hedgehog signals during hair follicle morphogenesis Liu, Ying Snedecor, Elizabeth R. Choi, Yeon Ja Yang, Ning Zhang, Xu Xu, Yuhuan Han, Yunlin Jones, Evan C. Shroyer, Kenneth R. Clark, Richard A. Zhang, Lianfeng Qin, Chuan Chen, Jiang Article GORAB is a golgin that localizes predominantly at the Golgi apparatus and physically interacts with small GTPases. GORAB is ubiquitously expressed in mammalian tissues, including the skin. However, the biological function of this golgin in skin is unknown. Here, we report that disrupting the expression of the Gorab gene in mice results in hair follicle morphogenesis defects that were characterized by impaired follicular keratinocyte differentiation. This hair follicle phenotype was associated with markedly suppressed hedgehog (Hh) signaling pathway in dermal condensates in vivo. Gorab-deficient dermal mesenchymal cells also displayed significantly reduced capability to respond to Hh pathway activation in vitro. Furthermore, we found that the formation of primary cilium, a cellular organelle that is essential for the Hh pathway, was impaired in mutant dermal papilla cells, suggesting that Gorab may be required for the Hh pathway through facilitating the formation of primary cilia. Thus, data obtained from this study provided insight onto the biological functions of Gorab during embryonic morphogenesis of skin in which Hh signaling and primary cilia exert important functions. 2015-11-24 2016-02 /pmc/articles/PMC4789774/ /pubmed/26967474 http://dx.doi.org/10.1016/j.jid.2015.10.067 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Liu, Ying Snedecor, Elizabeth R. Choi, Yeon Ja Yang, Ning Zhang, Xu Xu, Yuhuan Han, Yunlin Jones, Evan C. Shroyer, Kenneth R. Clark, Richard A. Zhang, Lianfeng Qin, Chuan Chen, Jiang |
| spellingShingle |
Liu, Ying Snedecor, Elizabeth R. Choi, Yeon Ja Yang, Ning Zhang, Xu Xu, Yuhuan Han, Yunlin Jones, Evan C. Shroyer, Kenneth R. Clark, Richard A. Zhang, Lianfeng Qin, Chuan Chen, Jiang Gorab is required for dermal condensate cells to respond to hedgehog signals during hair follicle morphogenesis |
| author_facet |
Liu, Ying Snedecor, Elizabeth R. Choi, Yeon Ja Yang, Ning Zhang, Xu Xu, Yuhuan Han, Yunlin Jones, Evan C. Shroyer, Kenneth R. Clark, Richard A. Zhang, Lianfeng Qin, Chuan Chen, Jiang |
| author_sort |
Liu, Ying |
| title |
Gorab is required for dermal condensate cells to
respond to hedgehog signals during hair follicle morphogenesis |
| title_short |
Gorab is required for dermal condensate cells to
respond to hedgehog signals during hair follicle morphogenesis |
| title_full |
Gorab is required for dermal condensate cells to
respond to hedgehog signals during hair follicle morphogenesis |
| title_fullStr |
Gorab is required for dermal condensate cells to
respond to hedgehog signals during hair follicle morphogenesis |
| title_full_unstemmed |
Gorab is required for dermal condensate cells to
respond to hedgehog signals during hair follicle morphogenesis |
| title_sort |
gorab is required for dermal condensate cells to
respond to hedgehog signals during hair follicle morphogenesis |
| description |
GORAB is a golgin that localizes predominantly at the Golgi apparatus and
physically interacts with small GTPases. GORAB is ubiquitously expressed in
mammalian tissues, including the skin. However, the biological function of this
golgin in skin is unknown. Here, we report that disrupting the expression of the
Gorab gene in mice results in hair follicle morphogenesis
defects that were characterized by impaired follicular keratinocyte
differentiation. This hair follicle phenotype was associated with markedly
suppressed hedgehog (Hh) signaling pathway in dermal condensates in
vivo. Gorab-deficient dermal mesenchymal cells
also displayed significantly reduced capability to respond to Hh pathway
activation in vitro. Furthermore, we found that the formation
of primary cilium, a cellular organelle that is essential for the Hh pathway,
was impaired in mutant dermal papilla cells, suggesting that
Gorab may be required for the Hh pathway through
facilitating the formation of primary cilia. Thus, data obtained from this study
provided insight onto the biological functions of Gorab during
embryonic morphogenesis of skin in which Hh signaling and primary cilia exert
important functions. |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4789774/ |
| _version_ |
1613551215443968000 |