Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus
Several studies have explored the origin and development mechanism of oral lichen planus (OLP) with limited attention to the role of bacteria in the progression of this common oral disease. Here we utilized MiSeq sequencing of 16S rRNA gene amplicons to identify complex oral microbiota associated wi...
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785528/ |
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pubmed-47855282016-03-11 Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus Wang, Kun Lu, Wenxin Tu, Qichao Ge, Yichen He, Jinzhi Zhou, Yu Gou, Yaping Van Nostrand, Joy D Qin, Yujia Li, Jiyao Zhou, Jizhong Li, Yan Xiao, Liying Zhou, Xuedong Article Several studies have explored the origin and development mechanism of oral lichen planus (OLP) with limited attention to the role of bacteria in the progression of this common oral disease. Here we utilized MiSeq sequencing of 16S rRNA gene amplicons to identify complex oral microbiota associated with OLP from saliva samples of two subtypes (reticular and erosive) of OLP patients and healthy controls. Our analyses indicated that the overall structure of the salivary microbiome was not significantly affected by disease status. However, we did observe evident variations in abundance for several taxonomic groups in OLP. Porphyromonas and Solobacterium showed significantly higher relative abundances, whereas Haemophilus, Corynebacterium, Cellulosimicrobium and Campylobacter showed lower abundances in OLP patients, as compared with healthy controls. In addition, we explored specific microbial co-occurrence patterns in OLP, and revealed significantly fewer linkers of Streptococcus comprising species in erosive OLP. Furthermore, the disease severity and immune dysregulation were also genus-associated, including with Porphyromonas that correlated to disease scores and salivary levels of interleukin (IL)-17 and IL-23. Overall, this study provides a general description of oral microbiome in OLP, and it will be useful for further investigation of their potential roles in the initiation and immune modulation of OLP. Nature Publishing Group 2016-03-10 /pmc/articles/PMC4785528/ /pubmed/26961389 http://dx.doi.org/10.1038/srep22943 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wang, Kun Lu, Wenxin Tu, Qichao Ge, Yichen He, Jinzhi Zhou, Yu Gou, Yaping Van Nostrand, Joy D Qin, Yujia Li, Jiyao Zhou, Jizhong Li, Yan Xiao, Liying Zhou, Xuedong |
| spellingShingle |
Wang, Kun Lu, Wenxin Tu, Qichao Ge, Yichen He, Jinzhi Zhou, Yu Gou, Yaping Van Nostrand, Joy D Qin, Yujia Li, Jiyao Zhou, Jizhong Li, Yan Xiao, Liying Zhou, Xuedong Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| author_facet |
Wang, Kun Lu, Wenxin Tu, Qichao Ge, Yichen He, Jinzhi Zhou, Yu Gou, Yaping Van Nostrand, Joy D Qin, Yujia Li, Jiyao Zhou, Jizhong Li, Yan Xiao, Liying Zhou, Xuedong |
| author_sort |
Wang, Kun |
| title |
Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| title_short |
Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| title_full |
Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| title_fullStr |
Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| title_full_unstemmed |
Preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| title_sort |
preliminary analysis of salivary microbiome and their potential roles in oral lichen planus |
| description |
Several studies have explored the origin and development mechanism of oral lichen planus (OLP) with limited attention to the role of bacteria in the progression of this common oral disease. Here we utilized MiSeq sequencing of 16S rRNA gene amplicons to identify complex oral microbiota associated with OLP from saliva samples of two subtypes (reticular and erosive) of OLP patients and healthy controls. Our analyses indicated that the overall structure of the salivary microbiome was not significantly affected by disease status. However, we did observe evident variations in abundance for several taxonomic groups in OLP. Porphyromonas and Solobacterium showed significantly higher relative abundances, whereas Haemophilus, Corynebacterium, Cellulosimicrobium and Campylobacter showed lower abundances in OLP patients, as compared with healthy controls. In addition, we explored specific microbial co-occurrence patterns in OLP, and revealed significantly fewer linkers of Streptococcus comprising species in erosive OLP. Furthermore, the disease severity and immune dysregulation were also genus-associated, including with Porphyromonas that correlated to disease scores and salivary levels of interleukin (IL)-17 and IL-23. Overall, this study provides a general description of oral microbiome in OLP, and it will be useful for further investigation of their potential roles in the initiation and immune modulation of OLP. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785528/ |
| _version_ |
1613549920107626496 |