Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse

The adaptive response to extreme endurance exercise might involve transcriptional and translational regulation by microRNAs (miRNAs). Therefore, the objective of the present study was to perform an integrated analysis of the blood transcriptome and miRNome (using microarrays) in the horse before and...

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Main Authors:	Mach, Núria, Plancade, Sandra, Pacholewska, Alicja, Lecardonnel, Jérôme, Rivière, Julie, Moroldo, Marco, Vaiman, Anne, Morgenthaler, Caroline, Beinat, Marine, Nevot, Alizée, Robert, Céline, Barrey, Eric
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785432/

id	pubmed-4785432
recordtype	oai_dc
spelling	pubmed-47854322016-03-11 Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse Mach, Núria Plancade, Sandra Pacholewska, Alicja Lecardonnel, Jérôme Rivière, Julie Moroldo, Marco Vaiman, Anne Morgenthaler, Caroline Beinat, Marine Nevot, Alizée Robert, Céline Barrey, Eric Article The adaptive response to extreme endurance exercise might involve transcriptional and translational regulation by microRNAs (miRNAs). Therefore, the objective of the present study was to perform an integrated analysis of the blood transcriptome and miRNome (using microarrays) in the horse before and after a 160 km endurance competition. A total of 2,453 differentially expressed genes and 167 differentially expressed microRNAs were identified when comparing pre- and post-ride samples. We used a hypergeometric test and its generalization to gain a better understanding of the biological functions regulated by the differentially expressed microRNA. In particular, 44 differentially expressed microRNAs putatively regulated a total of 351 depleted differentially expressed genes involved variously in glucose metabolism, fatty acid oxidation, mitochondrion biogenesis, and immune response pathways. In an independent validation set of animals, graphical Gaussian models confirmed that miR-21-5p, miR-181b-5p and miR-505-5p are candidate regulatory molecules for the adaptation to endurance exercise in the horse. To the best of our knowledge, the present study is the first to provide a comprehensive, integrated overview of the microRNA-mRNA co-regulation networks that may have a key role in controlling post-transcriptomic regulation during endurance exercise. Nature Publishing Group 2016-03-10 /pmc/articles/PMC4785432/ /pubmed/26960911 http://dx.doi.org/10.1038/srep22932 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Mach, Núria Plancade, Sandra Pacholewska, Alicja Lecardonnel, Jérôme Rivière, Julie Moroldo, Marco Vaiman, Anne Morgenthaler, Caroline Beinat, Marine Nevot, Alizée Robert, Céline Barrey, Eric
spellingShingle	Mach, Núria Plancade, Sandra Pacholewska, Alicja Lecardonnel, Jérôme Rivière, Julie Moroldo, Marco Vaiman, Anne Morgenthaler, Caroline Beinat, Marine Nevot, Alizée Robert, Céline Barrey, Eric Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
author_facet	Mach, Núria Plancade, Sandra Pacholewska, Alicja Lecardonnel, Jérôme Rivière, Julie Moroldo, Marco Vaiman, Anne Morgenthaler, Caroline Beinat, Marine Nevot, Alizée Robert, Céline Barrey, Eric
author_sort	Mach, Núria
title	Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
title_short	Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
title_full	Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
title_fullStr	Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
title_full_unstemmed	Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
title_sort	integrated mrna and mirna expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse
description	The adaptive response to extreme endurance exercise might involve transcriptional and translational regulation by microRNAs (miRNAs). Therefore, the objective of the present study was to perform an integrated analysis of the blood transcriptome and miRNome (using microarrays) in the horse before and after a 160 km endurance competition. A total of 2,453 differentially expressed genes and 167 differentially expressed microRNAs were identified when comparing pre- and post-ride samples. We used a hypergeometric test and its generalization to gain a better understanding of the biological functions regulated by the differentially expressed microRNA. In particular, 44 differentially expressed microRNAs putatively regulated a total of 351 depleted differentially expressed genes involved variously in glucose metabolism, fatty acid oxidation, mitochondrion biogenesis, and immune response pathways. In an independent validation set of animals, graphical Gaussian models confirmed that miR-21-5p, miR-181b-5p and miR-505-5p are candidate regulatory molecules for the adaptation to endurance exercise in the horse. To the best of our knowledge, the present study is the first to provide a comprehensive, integrated overview of the microRNA-mRNA co-regulation networks that may have a key role in controlling post-transcriptomic regulation during endurance exercise.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4785432/
_version_	1613549901544685568

Integrated mRNA and miRNA expression profiling in blood reveals candidate biomarkers associated with endurance exercise in the horse

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