Using selenomethionyl derivatives to assign sequence in low-resolution structures of the AP2 clathrin adaptor

A selenomethionine marker strategy allowed the identification of a region of disconnected electron density at low resolution and despite poor selenomethionine incorporation, thereby building a structural framework for understanding how the clathrin adaptor AP2 regulates clathrin binding in mammalian...

Full description

Bibliographic Details
Main Authors: Kelly, Bernard T., Graham, Stephen C., Owen, David J.
Format: Online
Language:English
Published: International Union of Crystallography 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4784665/