Robust normalization protocols for multiplexed fluorescence bioimage analysis

study of mapping and interaction of co-localized proteins at a sub-cellular level is important for understanding complex biological phenomena. One of the recent techniques to map co-localized proteins is to use the standard immuno-fluorescence microscopy in a cyclic manner (Nat Biotechnol 24:1270–8,...

Full description

Bibliographic Details
Main Authors: Ahmed Raza, Shan E, Langenkämper, Daniel, Sirinukunwattana, Korsuk, Epstein, David, Nattkemper, Tim W., Rajpoot, Nasir M.
Format: Online
Language:English
Published: BioMed Central 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4779207/