Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins
We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations th...
| Main Authors: | , , , , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Academic Press
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4771870/ |