Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples

Methanol-based fixation is superior to buffered formalin for next-generation sequencing of DNA from clinical cancer samples

High-quality tumour DNA is essential for any personalised treatment strategy based on NGS. Here we show that methanol fixation is superior to formalin, greater DNA yield, longer fragment size and more accurate copy-number calling using sWGS. We also show provide a new approach to understand fixation...

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Bibliographic Details
Main Authors: Piskorz, A. M., Ennis, D., Macintyre, G., Goranova, T. E., Eldridge, M., Segui-Gracia, N., Valganon, M., Hoyle, A., Orange, C., Moore, L., Jimenez-Linan, M., Millan, D., McNeish, I. A., Brenton, J. D.
Format: Online
Language:English
Published: Oxford University Press 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4769995/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4769995/

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