ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro

Cancer stem cells (CSCs) are associated with cancer recurrence and metastasis. Prostate cancer cells often metastasize to the bone with a complex microenvironment of cytokines favoring cell survival. In this study, the cell stemness influence of a group of interleukins including IL-3, 6, 10, 11 and...

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Main Authors:	Yu, Dandan, Zhong, Yali, Li, Xiaoran, Li, Yaqing, Li, Xiaoli, Cao, Jing, Fan, Huijie, Yuan, Yuan, Ji, Zhenyu, Qiao, Baoping, Wen, Jian-Guo, Zhang, Mingzhi, Kvalheim, Gunnar, Nesland, Jahn M., Suo, Zhenhe
Format:	Online
Language:	English
Published:	Impact Journals LLC 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767463/

id	pubmed-4767463
recordtype	oai_dc
spelling	pubmed-47674632016-03-25 ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro Yu, Dandan Zhong, Yali Li, Xiaoran Li, Yaqing Li, Xiaoli Cao, Jing Fan, Huijie Yuan, Yuan Ji, Zhenyu Qiao, Baoping Wen, Jian-Guo Zhang, Mingzhi Kvalheim, Gunnar Nesland, Jahn M. Suo, Zhenhe Research Paper Cancer stem cells (CSCs) are associated with cancer recurrence and metastasis. Prostate cancer cells often metastasize to the bone with a complex microenvironment of cytokines favoring cell survival. In this study, the cell stemness influence of a group of interleukins including IL-3, 6, 10, 11 and 24 on human prostate cancer cell lines LNCaP and PC-3 was explored in vitro. Sulforhodamine B(SRB) and 5-ethynyl-2′-deoxyuridine (EdU) assays were applied to examine the effect on cell proliferation, and wound healing and transwell assays were used for migration and invasion studies, in addition to colony formation, Western blotting and flowcytometry for the expression of stemness factors and chemotherapy sensitivity. We observed that ILs-3, 6 and 11 stimulated while ILs-10 and 24 inhibited the growth, invasion and migration of both cell lines. Interestingly, ILs-3, 6 and 11 significantly promoted colony formation and increased the expression of SOX2, CD44 and ABCG2 in both prostate cancer cell lines. However, ILs-10 and 24 showed the opposite effect on the expression of these factors. In line with the above findings, treatment with either IL-3 or IL-6 or IL-11 decreased the chemosensitivity to docetaxel while treatment with either IL-10 or IL-24 increased the sensitivity of docetaxel chemotherapy. In conclusion, our results suggest that ILs-3, 6 and 11 function as tumor promoters while ILs-10 and 24 function as tumor suppressors in the prostate cancer cell lines PC-3 and LNCaP in vitro, and such differences may attribute to their different effect on the stemness of PCa cells. Impact Journals LLC 2015-10-21 /pmc/articles/PMC4767463/ /pubmed/26528857 Text en Copyright: © 2015 Yu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yu, Dandan Zhong, Yali Li, Xiaoran Li, Yaqing Li, Xiaoli Cao, Jing Fan, Huijie Yuan, Yuan Ji, Zhenyu Qiao, Baoping Wen, Jian-Guo Zhang, Mingzhi Kvalheim, Gunnar Nesland, Jahn M. Suo, Zhenhe
spellingShingle	Yu, Dandan Zhong, Yali Li, Xiaoran Li, Yaqing Li, Xiaoli Cao, Jing Fan, Huijie Yuan, Yuan Ji, Zhenyu Qiao, Baoping Wen, Jian-Guo Zhang, Mingzhi Kvalheim, Gunnar Nesland, Jahn M. Suo, Zhenhe ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro
author_facet	Yu, Dandan Zhong, Yali Li, Xiaoran Li, Yaqing Li, Xiaoli Cao, Jing Fan, Huijie Yuan, Yuan Ji, Zhenyu Qiao, Baoping Wen, Jian-Guo Zhang, Mingzhi Kvalheim, Gunnar Nesland, Jahn M. Suo, Zhenhe
author_sort	Yu, Dandan
title	ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro
title_short	ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro
title_full	ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro
title_fullStr	ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro
title_full_unstemmed	ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro
title_sort	ils-3, 6 and 11 increase, but ils-10 and 24 decrease stemness of human prostate cancer cells in vitro
description	Cancer stem cells (CSCs) are associated with cancer recurrence and metastasis. Prostate cancer cells often metastasize to the bone with a complex microenvironment of cytokines favoring cell survival. In this study, the cell stemness influence of a group of interleukins including IL-3, 6, 10, 11 and 24 on human prostate cancer cell lines LNCaP and PC-3 was explored in vitro. Sulforhodamine B(SRB) and 5-ethynyl-2′-deoxyuridine (EdU) assays were applied to examine the effect on cell proliferation, and wound healing and transwell assays were used for migration and invasion studies, in addition to colony formation, Western blotting and flowcytometry for the expression of stemness factors and chemotherapy sensitivity. We observed that ILs-3, 6 and 11 stimulated while ILs-10 and 24 inhibited the growth, invasion and migration of both cell lines. Interestingly, ILs-3, 6 and 11 significantly promoted colony formation and increased the expression of SOX2, CD44 and ABCG2 in both prostate cancer cell lines. However, ILs-10 and 24 showed the opposite effect on the expression of these factors. In line with the above findings, treatment with either IL-3 or IL-6 or IL-11 decreased the chemosensitivity to docetaxel while treatment with either IL-10 or IL-24 increased the sensitivity of docetaxel chemotherapy. In conclusion, our results suggest that ILs-3, 6 and 11 function as tumor promoters while ILs-10 and 24 function as tumor suppressors in the prostate cancer cell lines PC-3 and LNCaP in vitro, and such differences may attribute to their different effect on the stemness of PCa cells.
publisher	Impact Journals LLC
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4767463/
_version_	1613543395961077760

ILs-3, 6 and 11 increase, but ILs-10 and 24 decrease stemness of human prostate cancer cells in vitro

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