Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor

Circular RNAs (circRNAs) are widespread circles of non-coding RNAs with largely unknown function. Because stimulation of mammary cells with the epidermal growth factor (EGF) leads to dynamic changes in the abundance of coding and non-coding RNA molecules, and culminates in the acquisition of a robus...

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Main Authors:	Enuka, Yehoshua, Lauriola, Mattia, Feldman, Morris E., Sas-Chen, Aldema, Ulitsky, Igor, Yarden, Yosef
Format:	Online
Language:	English
Published:	Oxford University Press 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756822/

id	pubmed-4756822
recordtype	oai_dc
spelling	pubmed-47568222016-02-18 Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor Enuka, Yehoshua Lauriola, Mattia Feldman, Morris E. Sas-Chen, Aldema Ulitsky, Igor Yarden, Yosef RNA Circular RNAs (circRNAs) are widespread circles of non-coding RNAs with largely unknown function. Because stimulation of mammary cells with the epidermal growth factor (EGF) leads to dynamic changes in the abundance of coding and non-coding RNA molecules, and culminates in the acquisition of a robust migratory phenotype, this cellular model might disclose functions of circRNAs. Here we show that circRNAs of EGF-stimulated mammary cells are stably expressed, while mRNAs and microRNAs change within minutes. In general, the circRNAs we detected are relatively long-lived and weakly expressed. Interestingly, they are almost ubiquitously co-expressed with the corresponding linear transcripts, and the respective, shared promoter regions are more active compared to genes producing linear isoforms with no detectable circRNAs. These findings imply that altered abundance of circRNAs, unlike changes in the levels of other RNAs, might not play critical roles in signaling cascades and downstream transcriptional networks that rapidly commit cells to specific outcomes. Oxford University Press 2016-02-18 2015-12-10 /pmc/articles/PMC4756822/ /pubmed/26657629 http://dx.doi.org/10.1093/nar/gkv1367 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Enuka, Yehoshua Lauriola, Mattia Feldman, Morris E. Sas-Chen, Aldema Ulitsky, Igor Yarden, Yosef
spellingShingle	Enuka, Yehoshua Lauriola, Mattia Feldman, Morris E. Sas-Chen, Aldema Ulitsky, Igor Yarden, Yosef Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
author_facet	Enuka, Yehoshua Lauriola, Mattia Feldman, Morris E. Sas-Chen, Aldema Ulitsky, Igor Yarden, Yosef
author_sort	Enuka, Yehoshua
title	Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
title_short	Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
title_full	Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
title_fullStr	Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
title_full_unstemmed	Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor
title_sort	circular rnas are long-lived and display only minimal early alterations in response to a growth factor
description	Circular RNAs (circRNAs) are widespread circles of non-coding RNAs with largely unknown function. Because stimulation of mammary cells with the epidermal growth factor (EGF) leads to dynamic changes in the abundance of coding and non-coding RNA molecules, and culminates in the acquisition of a robust migratory phenotype, this cellular model might disclose functions of circRNAs. Here we show that circRNAs of EGF-stimulated mammary cells are stably expressed, while mRNAs and microRNAs change within minutes. In general, the circRNAs we detected are relatively long-lived and weakly expressed. Interestingly, they are almost ubiquitously co-expressed with the corresponding linear transcripts, and the respective, shared promoter regions are more active compared to genes producing linear isoforms with no detectable circRNAs. These findings imply that altered abundance of circRNAs, unlike changes in the levels of other RNAs, might not play critical roles in signaling cascades and downstream transcriptional networks that rapidly commit cells to specific outcomes.
publisher	Oxford University Press
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756822/
_version_	1613539841616642048

Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor

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