Phenotypic and functional alterations of pDCs in lupus-prone mice

Plasmacytoid dendritic cells (pDCs) were considered to be the major IFNα source in systemic lupus erythematosus (SLE) but their phenotype and function in different disease status have not been well studied. To study the function and phenotype of pDCs in lupus-prone mice we used 7 strains of lupus-pr...

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Main Authors:	Zhou, Zhenyuan, Ma, Jianyang, Xiao, Chunyuan, Han, Xiao, Qiu, Rong, Wang, Yan, Zhou, Yingying, Wu, Li, Huang, Xinfang, Shen, Nan
Format:	Online
Language:	English
Published:	Nature Publishing Group 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754657/

id	pubmed-4754657
recordtype	oai_dc
spelling	pubmed-47546572016-02-24 Phenotypic and functional alterations of pDCs in lupus-prone mice Zhou, Zhenyuan Ma, Jianyang Xiao, Chunyuan Han, Xiao Qiu, Rong Wang, Yan Zhou, Yingying Wu, Li Huang, Xinfang Shen, Nan Article Plasmacytoid dendritic cells (pDCs) were considered to be the major IFNα source in systemic lupus erythematosus (SLE) but their phenotype and function in different disease status have not been well studied. To study the function and phenotype of pDCs in lupus-prone mice we used 7 strains of lupus-prone mice including NZB/W F1, NZB, NZW, NZM2410, B6.NZMSle1/2/3, MRL/lpr and BXSB/Mp mice and C57BL/6 as control mice. Increased spleen pDC numbers were found in most lupus mice compared to C57BL/6 mice. The IFNα-producing ability of BM pDCs was similar between lupus and C57BL/6 mice, whereas pDCs from the spleens of NZB/W F1 and NZB mice produced more IFNα than pDCs from the spleens of C57BL/6 mice. Furthermore, spleen pDCs from MRL-lpr and NZM2410 mice showed increased responses to Tlr7 and Tlr9, respectively. As the disease progressed, IFN signature were evaluated in both BM and spleen pDC from lupus prone mice and the number of BM pDCs and their ability to produce IFNα gradually decreased in lupus-prone mice. In conclusion, pDC are activated alone with disease development and its phenotype and function differ among lupus-prone strains, and these differences may contribute to the development of lupus in these mice. Nature Publishing Group 2016-02-16 /pmc/articles/PMC4754657/ /pubmed/26879679 http://dx.doi.org/10.1038/srep20373 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhou, Zhenyuan Ma, Jianyang Xiao, Chunyuan Han, Xiao Qiu, Rong Wang, Yan Zhou, Yingying Wu, Li Huang, Xinfang Shen, Nan
spellingShingle	Zhou, Zhenyuan Ma, Jianyang Xiao, Chunyuan Han, Xiao Qiu, Rong Wang, Yan Zhou, Yingying Wu, Li Huang, Xinfang Shen, Nan Phenotypic and functional alterations of pDCs in lupus-prone mice
author_facet	Zhou, Zhenyuan Ma, Jianyang Xiao, Chunyuan Han, Xiao Qiu, Rong Wang, Yan Zhou, Yingying Wu, Li Huang, Xinfang Shen, Nan
author_sort	Zhou, Zhenyuan
title	Phenotypic and functional alterations of pDCs in lupus-prone mice
title_short	Phenotypic and functional alterations of pDCs in lupus-prone mice
title_full	Phenotypic and functional alterations of pDCs in lupus-prone mice
title_fullStr	Phenotypic and functional alterations of pDCs in lupus-prone mice
title_full_unstemmed	Phenotypic and functional alterations of pDCs in lupus-prone mice
title_sort	phenotypic and functional alterations of pdcs in lupus-prone mice
description	Plasmacytoid dendritic cells (pDCs) were considered to be the major IFNα source in systemic lupus erythematosus (SLE) but their phenotype and function in different disease status have not been well studied. To study the function and phenotype of pDCs in lupus-prone mice we used 7 strains of lupus-prone mice including NZB/W F1, NZB, NZW, NZM2410, B6.NZMSle1/2/3, MRL/lpr and BXSB/Mp mice and C57BL/6 as control mice. Increased spleen pDC numbers were found in most lupus mice compared to C57BL/6 mice. The IFNα-producing ability of BM pDCs was similar between lupus and C57BL/6 mice, whereas pDCs from the spleens of NZB/W F1 and NZB mice produced more IFNα than pDCs from the spleens of C57BL/6 mice. Furthermore, spleen pDCs from MRL-lpr and NZM2410 mice showed increased responses to Tlr7 and Tlr9, respectively. As the disease progressed, IFN signature were evaluated in both BM and spleen pDC from lupus prone mice and the number of BM pDCs and their ability to produce IFNα gradually decreased in lupus-prone mice. In conclusion, pDC are activated alone with disease development and its phenotype and function differ among lupus-prone strains, and these differences may contribute to the development of lupus in these mice.
publisher	Nature Publishing Group
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4754657/
_version_	1613538978337652736

Phenotypic and functional alterations of pDCs in lupus-prone mice

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