Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa
Pyocyanin (PCN), a virulence factor synthesized by Pseudomonas aeruginosa, plays an important role during clinical infections. There is no study of the effect of nitric oxide (NO) on PCN biosynthesis. Here, the effect of NO on PCN levels in Pseudomonas aeruginosa strain PAO1, a common reference stra...
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| Format: | Online |
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Elsevier
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753387/ |
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pubmed-4753387 |
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pubmed-47533872016-03-02 Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa Gao, Lei Zhang, Yuying Wang, Yan Qiao, Xinhua Zi, Jing Chen, Chang Wan, Yi Research Paper Pyocyanin (PCN), a virulence factor synthesized by Pseudomonas aeruginosa, plays an important role during clinical infections. There is no study of the effect of nitric oxide (NO) on PCN biosynthesis. Here, the effect of NO on PCN levels in Pseudomonas aeruginosa strain PAO1, a common reference strain, was tested. The results showed that the NO donor sodium nitroprusside (SNP) can significantly reduce PCN levels (82.5% reduction at 60 μM SNP). Furthermore, the effect of endogenous NO on PCN was tested by constructing PAO1 nor (NO reductase gene) knockout mutants. Compared to the wild-type strain, the Δnor strain had a lower PCN (86% reduction in Δnor). To examine whether the results were universal with other P. aeruginosa strains, we collected 4 clinical strains from a hospital, tested their PCN levels after SNP treatment, and obtained similar results, i.e., PCN biosynthesis was inhibited by NO. These results suggest that NO treatment may be a new strategy to inhibit PCN biosynthesis and could provide novel insights into eliminating P. aeruginosa virulence as a clinical goal. Elsevier 2015-11-06 /pmc/articles/PMC4753387/ /pubmed/26874276 http://dx.doi.org/10.1016/j.redox.2015.10.005 Text en © 2016 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Gao, Lei Zhang, Yuying Wang, Yan Qiao, Xinhua Zi, Jing Chen, Chang Wan, Yi |
| spellingShingle |
Gao, Lei Zhang, Yuying Wang, Yan Qiao, Xinhua Zi, Jing Chen, Chang Wan, Yi Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa |
| author_facet |
Gao, Lei Zhang, Yuying Wang, Yan Qiao, Xinhua Zi, Jing Chen, Chang Wan, Yi |
| author_sort |
Gao, Lei |
| title |
Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa |
| title_short |
Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa |
| title_full |
Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa |
| title_fullStr |
Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa |
| title_full_unstemmed |
Reduction of PCN biosynthesis by NO in Pseudomonas aeruginosa |
| title_sort |
reduction of pcn biosynthesis by no in pseudomonas aeruginosa |
| description |
Pyocyanin (PCN), a virulence factor synthesized by Pseudomonas aeruginosa, plays an important role during clinical infections. There is no study of the effect of nitric oxide (NO) on PCN biosynthesis. Here, the effect of NO on PCN levels in Pseudomonas aeruginosa strain PAO1, a common reference strain, was tested. The results showed that the NO donor sodium nitroprusside (SNP) can significantly reduce PCN levels (82.5% reduction at 60 μM SNP). Furthermore, the effect of endogenous NO on PCN was tested by constructing PAO1 nor (NO reductase gene) knockout mutants. Compared to the wild-type strain, the Δnor strain had a lower PCN (86% reduction in Δnor). To examine whether the results were universal with other P. aeruginosa strains, we collected 4 clinical strains from a hospital, tested their PCN levels after SNP treatment, and obtained similar results, i.e., PCN biosynthesis was inhibited by NO. These results suggest that NO treatment may be a new strategy to inhibit PCN biosynthesis and could provide novel insights into eliminating P. aeruginosa virulence as a clinical goal. |
| publisher |
Elsevier |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753387/ |
| _version_ |
1613538451194380288 |