Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma

WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) has been speculated to play important roles in the development of several kinds of cancers. However, the role of WWP1 in hepatocellular carcinoma(HCC) is not clear. In the present study, we investigated the expression and prognostic role of W...

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Main Authors:	Zhang, Xiao-Fei, Chao, Jie, Pan, Qiu-Zhong, Pan, Ke, Weng, De-Sheng, Wang, Qi-Jing, Zhao, Jing-Jing, He, Jia, Liu, Qing, Jiang, Shan-Shan, Chen, Chang-Long, Zhang, Hong-Xia, Xia, Jian-Chuan
Format:	Online
Language:	English
Published:	Impact Journals LLC 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747378/

id	pubmed-4747378
recordtype	oai_dc
spelling	pubmed-47473782016-03-24 Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma Zhang, Xiao-Fei Chao, Jie Pan, Qiu-Zhong Pan, Ke Weng, De-Sheng Wang, Qi-Jing Zhao, Jing-Jing He, Jia Liu, Qing Jiang, Shan-Shan Chen, Chang-Long Zhang, Hong-Xia Xia, Jian-Chuan Research Paper WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) has been speculated to play important roles in the development of several kinds of cancers. However, the role of WWP1 in hepatocellular carcinoma(HCC) is not clear. In the present study, we investigated the expression and prognostic role of WWP1 in primary hepatocellular carcinoma (HCC) using cell lines and 149 archived HCC samples. Correlation between the functions of WWP1 in HCC was also explored. We used human HCC cell lines (BEL-7402, SMMC-7721, Hep-G2, Hep-3B, SK-hep1 and Huh7) and a normal hepatocyte cell line (LO2) along with HCC samples from patients who had undergone resection for HCC previously at our hospital. A battery of methods (real-time quantitative polymerase chain reaction; western blotting; immunohistochemical analyses; cell proliferation and colony formation assays; cell migration and cell invasion assays) were employed to assess various aspects of WWP1. We found that WWP1 expression was upregulated aberrantly at mRNA and protein levels in human primary HCC tissues. Amplified expression of WWP1 was highly correlated with poor outcome. Silencing of WWP1 expression by siRNA inhibited the proliferation, colony formation, migration and invasion of HCC cells in vitro, and resulted in significant apoptosis and cycle arrest in HCC cells. Our findings suggest that WWP1 might have an oncogenic role in human primary HCC, and that it could be used as a prognostic marker as well as a potential molecular target for the treatment of HCC. Impact Journals LLC 2015-10-17 /pmc/articles/PMC4747378/ /pubmed/26506518 Text en Copyright: © 2015 Zhang et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Zhang, Xiao-Fei Chao, Jie Pan, Qiu-Zhong Pan, Ke Weng, De-Sheng Wang, Qi-Jing Zhao, Jing-Jing He, Jia Liu, Qing Jiang, Shan-Shan Chen, Chang-Long Zhang, Hong-Xia Xia, Jian-Chuan
spellingShingle	Zhang, Xiao-Fei Chao, Jie Pan, Qiu-Zhong Pan, Ke Weng, De-Sheng Wang, Qi-Jing Zhao, Jing-Jing He, Jia Liu, Qing Jiang, Shan-Shan Chen, Chang-Long Zhang, Hong-Xia Xia, Jian-Chuan Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
author_facet	Zhang, Xiao-Fei Chao, Jie Pan, Qiu-Zhong Pan, Ke Weng, De-Sheng Wang, Qi-Jing Zhao, Jing-Jing He, Jia Liu, Qing Jiang, Shan-Shan Chen, Chang-Long Zhang, Hong-Xia Xia, Jian-Chuan
author_sort	Zhang, Xiao-Fei
title	Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
title_short	Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
title_full	Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
title_fullStr	Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
title_full_unstemmed	Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
title_sort	overexpression of wwp1 promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma
description	WW domain-containing E3 ubiquitin protein ligase 1 (WWP1) has been speculated to play important roles in the development of several kinds of cancers. However, the role of WWP1 in hepatocellular carcinoma(HCC) is not clear. In the present study, we investigated the expression and prognostic role of WWP1 in primary hepatocellular carcinoma (HCC) using cell lines and 149 archived HCC samples. Correlation between the functions of WWP1 in HCC was also explored. We used human HCC cell lines (BEL-7402, SMMC-7721, Hep-G2, Hep-3B, SK-hep1 and Huh7) and a normal hepatocyte cell line (LO2) along with HCC samples from patients who had undergone resection for HCC previously at our hospital. A battery of methods (real-time quantitative polymerase chain reaction; western blotting; immunohistochemical analyses; cell proliferation and colony formation assays; cell migration and cell invasion assays) were employed to assess various aspects of WWP1. We found that WWP1 expression was upregulated aberrantly at mRNA and protein levels in human primary HCC tissues. Amplified expression of WWP1 was highly correlated with poor outcome. Silencing of WWP1 expression by siRNA inhibited the proliferation, colony formation, migration and invasion of HCC cells in vitro, and resulted in significant apoptosis and cycle arrest in HCC cells. Our findings suggest that WWP1 might have an oncogenic role in human primary HCC, and that it could be used as a prognostic marker as well as a potential molecular target for the treatment of HCC.
publisher	Impact Journals LLC
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4747378/
_version_	1613536211342721024

Overexpression of WWP1 Promotes tumorigenesis and predicts unfavorable prognosis in patients with hepatocellular carcinoma

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