GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex
GABAergic interneurons play key roles in cortical circuits, yet little is known about their early connectivity. Here we use glutamate uncaging and a novel optogenetic strategy to track changes in the afferent and efferent synaptic connections of developing neocortical interneuron subtypes. We find t...
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743032/ |
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pubmed-47430322016-03-04 GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex Anastasiades, Paul G. Marques-Smith, Andre Lyngholm, Daniel Lickiss, Tom Raffiq, Sayda Kätzel, Dennis Miesenböck, Gero Butt, Simon J. B. Article GABAergic interneurons play key roles in cortical circuits, yet little is known about their early connectivity. Here we use glutamate uncaging and a novel optogenetic strategy to track changes in the afferent and efferent synaptic connections of developing neocortical interneuron subtypes. We find that Nkx2-1-derived interneurons possess functional synaptic connections before emerging pyramidal cell networks. Subsequent interneuron circuit maturation is both subtype and layer dependent. Glutamatergic input onto fast spiking (FS), but not somatostatin-positive, non-FS interneurons increases over development. Interneurons of both subtype located in layers (L) 4 and 5b engage in transient circuits that disappear after the somatosensory critical period. These include a pathway mediated by L5b somatostatin-positive interneurons that specifically targets L4 during the first postnatal week. The innervation patterns of immature cortical interneuron circuits are thus neither static nor progressively strengthened but follow a layer-specific choreography of transient connections that differ from those of the adult brain. Nature Publishing Group 2016-02-04 /pmc/articles/PMC4743032/ /pubmed/26843463 http://dx.doi.org/10.1038/ncomms10584 Text en Copyright © 2016, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Anastasiades, Paul G. Marques-Smith, Andre Lyngholm, Daniel Lickiss, Tom Raffiq, Sayda Kätzel, Dennis Miesenböck, Gero Butt, Simon J. B. |
| spellingShingle |
Anastasiades, Paul G. Marques-Smith, Andre Lyngholm, Daniel Lickiss, Tom Raffiq, Sayda Kätzel, Dennis Miesenböck, Gero Butt, Simon J. B. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| author_facet |
Anastasiades, Paul G. Marques-Smith, Andre Lyngholm, Daniel Lickiss, Tom Raffiq, Sayda Kätzel, Dennis Miesenböck, Gero Butt, Simon J. B. |
| author_sort |
Anastasiades, Paul G. |
| title |
GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| title_short |
GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| title_full |
GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| title_fullStr |
GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| title_full_unstemmed |
GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| title_sort |
gabaergic interneurons form transient layer-specific circuits in early postnatal neocortex |
| description |
GABAergic interneurons play key roles in cortical circuits, yet little is known about their early connectivity. Here we use glutamate uncaging and a novel optogenetic strategy to track changes in the afferent and efferent synaptic connections of developing neocortical interneuron subtypes. We find that Nkx2-1-derived interneurons possess functional synaptic connections before emerging pyramidal cell networks. Subsequent interneuron circuit maturation is both subtype and layer dependent. Glutamatergic input onto fast spiking (FS), but not somatostatin-positive, non-FS interneurons increases over development. Interneurons of both subtype located in layers (L) 4 and 5b engage in transient circuits that disappear after the somatosensory critical period. These include a pathway mediated by L5b somatostatin-positive interneurons that specifically targets L4 during the first postnatal week. The innervation patterns of immature cortical interneuron circuits are thus neither static nor progressively strengthened but follow a layer-specific choreography of transient connections that differ from those of the adult brain. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743032/ |
| _version_ |
1613534487568711680 |