Isolation and characterization of novel RECK tumor suppressor gene splice variants

Glioblastoma multiforme is the most common and lethal of the central nervous system glial-derived tumors. RECK suppresses tumor invasion by negatively regulating at least three members of the matrix metalloproteinase family: MMP-9, MMP-2, and MT1-MMP. A positive correlation has been observed between...

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Main Authors:	Trombetta-Lima, Marina, Winnischofer, Sheila Maria Brochado, Demasi, Marcos Angelo Almeida, Filho, Renato Astorino, Carreira, Ana Claudia Oliveira, Wei, Beiyang, de Assis Ribas, Thais, Konig, Michelle Silberspitz, Bowman-Colin, Christian, Oba-Shinjo, Sueli Mieko, Marie, Suely Kazue Nagahashi, Stetler-Stevenson, William, Sogayar, Mari Cleide
Format:	Online
Language:	English
Published:	Impact Journals LLC 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741753/

id	pubmed-4741753
recordtype	oai_dc
spelling	pubmed-47417532016-03-11 Isolation and characterization of novel RECK tumor suppressor gene splice variants Trombetta-Lima, Marina Winnischofer, Sheila Maria Brochado Demasi, Marcos Angelo Almeida Filho, Renato Astorino Carreira, Ana Claudia Oliveira Wei, Beiyang de Assis Ribas, Thais Konig, Michelle Silberspitz Bowman-Colin, Christian Oba-Shinjo, Sueli Mieko Marie, Suely Kazue Nagahashi Stetler-Stevenson, William Sogayar, Mari Cleide Research Paper Glioblastoma multiforme is the most common and lethal of the central nervous system glial-derived tumors. RECK suppresses tumor invasion by negatively regulating at least three members of the matrix metalloproteinase family: MMP-9, MMP-2, and MT1-MMP. A positive correlation has been observed between the abundance of RECK expression in tumor samples and a more favorable prognosis for patients with several types of tumors. In the present study, novel alternatively spliced variants of the RECK gene: RECK-B and RECK-I were isolated by RT-PCR and sequenced. The expression levels and profiles of these alternative RECK transcripts, as well as canonical RECK were determined in tissue samples of malignant astrocytomas of different grades and in a normal tissue RNA panel by qRT-PCR. Our results show that higher canonical RECK expression, accompanied by a higher canonical to alternative transcript expression ratio, positively correlates with higher overall survival rate after chemotherapeutic treatment of GBM patients. U87MG and T98G cells over-expressing the RECK-B alternative variant display higher anchorage-independent clonal growth and do not display modulation of, respectively, MMP-2 and MMP-9 expression. Our findings suggest that RECK transcript variants might have opposite roles in GBM biology and the ratio of their expression levels may be informative for the prognostic outcome of GBM patients. Impact Journals LLC 2015-09-28 /pmc/articles/PMC4741753/ /pubmed/26431549 Text en Copyright: © 2015 Trombetta-Lima et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Trombetta-Lima, Marina Winnischofer, Sheila Maria Brochado Demasi, Marcos Angelo Almeida Filho, Renato Astorino Carreira, Ana Claudia Oliveira Wei, Beiyang de Assis Ribas, Thais Konig, Michelle Silberspitz Bowman-Colin, Christian Oba-Shinjo, Sueli Mieko Marie, Suely Kazue Nagahashi Stetler-Stevenson, William Sogayar, Mari Cleide
spellingShingle	Trombetta-Lima, Marina Winnischofer, Sheila Maria Brochado Demasi, Marcos Angelo Almeida Filho, Renato Astorino Carreira, Ana Claudia Oliveira Wei, Beiyang de Assis Ribas, Thais Konig, Michelle Silberspitz Bowman-Colin, Christian Oba-Shinjo, Sueli Mieko Marie, Suely Kazue Nagahashi Stetler-Stevenson, William Sogayar, Mari Cleide Isolation and characterization of novel RECK tumor suppressor gene splice variants
author_facet	Trombetta-Lima, Marina Winnischofer, Sheila Maria Brochado Demasi, Marcos Angelo Almeida Filho, Renato Astorino Carreira, Ana Claudia Oliveira Wei, Beiyang de Assis Ribas, Thais Konig, Michelle Silberspitz Bowman-Colin, Christian Oba-Shinjo, Sueli Mieko Marie, Suely Kazue Nagahashi Stetler-Stevenson, William Sogayar, Mari Cleide
author_sort	Trombetta-Lima, Marina
title	Isolation and characterization of novel RECK tumor suppressor gene splice variants
title_short	Isolation and characterization of novel RECK tumor suppressor gene splice variants
title_full	Isolation and characterization of novel RECK tumor suppressor gene splice variants
title_fullStr	Isolation and characterization of novel RECK tumor suppressor gene splice variants
title_full_unstemmed	Isolation and characterization of novel RECK tumor suppressor gene splice variants
title_sort	isolation and characterization of novel reck tumor suppressor gene splice variants
description	Glioblastoma multiforme is the most common and lethal of the central nervous system glial-derived tumors. RECK suppresses tumor invasion by negatively regulating at least three members of the matrix metalloproteinase family: MMP-9, MMP-2, and MT1-MMP. A positive correlation has been observed between the abundance of RECK expression in tumor samples and a more favorable prognosis for patients with several types of tumors. In the present study, novel alternatively spliced variants of the RECK gene: RECK-B and RECK-I were isolated by RT-PCR and sequenced. The expression levels and profiles of these alternative RECK transcripts, as well as canonical RECK were determined in tissue samples of malignant astrocytomas of different grades and in a normal tissue RNA panel by qRT-PCR. Our results show that higher canonical RECK expression, accompanied by a higher canonical to alternative transcript expression ratio, positively correlates with higher overall survival rate after chemotherapeutic treatment of GBM patients. U87MG and T98G cells over-expressing the RECK-B alternative variant display higher anchorage-independent clonal growth and do not display modulation of, respectively, MMP-2 and MMP-9 expression. Our findings suggest that RECK transcript variants might have opposite roles in GBM biology and the ratio of their expression levels may be informative for the prognostic outcome of GBM patients.
publisher	Impact Journals LLC
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741753/
_version_	1613533903069380608

Isolation and characterization of novel RECK tumor suppressor gene splice variants

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