Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees

To clarify the mechanisms of Nosema ceranae parasitism, we deep-sequenced both honey bee host and parasite mRNAs throughout a complete 6-day infection cycle. By time-series analysis, 1122 parasite genes were significantly differently expressed during the reproduction cycle, clustering into 4 express...

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Main Authors:	Huang, Qiang, Chen, Yan Ping, Wang, Rui Wu, Cheng, Shang, Evans, Jay D.
Format:	Online
Language:	English
Published:	Public Library of Science 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739525/

id	pubmed-4739525
recordtype	oai_dc
spelling	pubmed-47395252016-02-11 Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees Huang, Qiang Chen, Yan Ping Wang, Rui Wu Cheng, Shang Evans, Jay D. Research Article To clarify the mechanisms of Nosema ceranae parasitism, we deep-sequenced both honey bee host and parasite mRNAs throughout a complete 6-day infection cycle. By time-series analysis, 1122 parasite genes were significantly differently expressed during the reproduction cycle, clustering into 4 expression patterns. We found reactive mitochondrial oxygen species modulator 1 of the host to be significantly down regulated during the entire infection period. Our data support the hypothesis that apoptosis of honey bee cells was suppressed during infection. We further analyzed genome-wide genetic diversity of this parasite by comparing samples collected from the same site in 2007 and 2013. The number of SNP positions per gene and the proportion of non-synonymous substitutions per gene were significantly reduced over this time period, suggesting purifying selection on the parasite genome and supporting the hypothesis that a subset of N. ceranae strains might be dominating infection. Public Library of Science 2016-02-03 /pmc/articles/PMC4739525/ /pubmed/26840596 http://dx.doi.org/10.1371/journal.pone.0147549 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Huang, Qiang Chen, Yan Ping Wang, Rui Wu Cheng, Shang Evans, Jay D.
spellingShingle	Huang, Qiang Chen, Yan Ping Wang, Rui Wu Cheng, Shang Evans, Jay D. Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
author_facet	Huang, Qiang Chen, Yan Ping Wang, Rui Wu Cheng, Shang Evans, Jay D.
author_sort	Huang, Qiang
title	Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
title_short	Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
title_full	Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
title_fullStr	Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
title_full_unstemmed	Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees
title_sort	host-parasite interactions and purifying selection in a microsporidian parasite of honey bees
description	To clarify the mechanisms of Nosema ceranae parasitism, we deep-sequenced both honey bee host and parasite mRNAs throughout a complete 6-day infection cycle. By time-series analysis, 1122 parasite genes were significantly differently expressed during the reproduction cycle, clustering into 4 expression patterns. We found reactive mitochondrial oxygen species modulator 1 of the host to be significantly down regulated during the entire infection period. Our data support the hypothesis that apoptosis of honey bee cells was suppressed during infection. We further analyzed genome-wide genetic diversity of this parasite by comparing samples collected from the same site in 2007 and 2013. The number of SNP positions per gene and the proportion of non-synonymous substitutions per gene were significantly reduced over this time period, suggesting purifying selection on the parasite genome and supporting the hypothesis that a subset of N. ceranae strains might be dominating infection.
publisher	Public Library of Science
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739525/
_version_	1613533144817860608

Host-Parasite Interactions and Purifying Selection in a Microsporidian Parasite of Honey Bees

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