Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients

Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients

Rapid and accurate diagnosis of pulmonary tuberculosis (PTB) is an unresolved problem worldwide, especially for sputum smear− (S−) cases. In this study, five antigen genes including Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 were cloned from Mycobacterium tuberculosis (Mtb) RD1 and overexpressed to...

Full description

Bibliographic Details
Main Authors: Liu, Zhiqiang, Qie, Shuang, Li, Lili, Xiu, Bingshui, Yang, Xiqin, Dai, Zhenhua, Zhang, Xuhui, Duan, Cuimi, Que, Haiping, Zhao, Ping, Johnson, Heather, Zhang, Heqiu, Feng, Xiaoyan
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739478/
id pubmed-4739478
recordtype oai_dc
spelling pubmed-47394782016-02-16 Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients Liu, Zhiqiang Qie, Shuang Li, Lili Xiu, Bingshui Yang, Xiqin Dai, Zhenhua Zhang, Xuhui Duan, Cuimi Que, Haiping Zhao, Ping Johnson, Heather Zhang, Heqiu Feng, Xiaoyan Research Article Rapid and accurate diagnosis of pulmonary tuberculosis (PTB) is an unresolved problem worldwide, especially for sputum smear− (S−) cases. In this study, five antigen genes including Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 were cloned from Mycobacterium tuberculosis (Mtb) RD1 and overexpressed to generate antigen fragments. These antigens and their combinations were investigated for PTB serodiagnosis. 298 serum samples were collected from active PTB patients, including 117 sputum smear+ (S+) and sputum culture+ (C+) cases, 101 S−/C+ cases, and 80 S−/C− cases. The serum IgG levels of the five antigens were measured by ELISA. Based on IgG levels, the sensitivity/specificity of Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 for PTB detection was 81.21%/74.74%, 63.09%/94.78%, 32.21%/87.37%, 62.42%/85.26%, and 83.56%/83.16%, respectively. Furthermore, the optimal result for PTB diagnosis was achieved by combining antigens Rv3871, Rv3876, and Rv3879. In addition, the IgG levels of Rv3871, Rv3876, and Rv3879 were found to be higher in S−/C+ PTB patients than in other PTB populations. More importantly, combination of the three antigens demonstrated superior diagnostic performance for both S−/C+ and S−/C− PTB. In conclusion, the combination of Rv3871, Rv3876, and Rv3879 induced higher IgG response in sputum S−/C+ PTB patients and represents a promising biomarker combination for diagnosing of PTB. Hindawi Publishing Corporation 2016 2016-01-18 /pmc/articles/PMC4739478/ /pubmed/26885516 http://dx.doi.org/10.1155/2016/7486425 Text en Copyright © 2016 Zhiqiang Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Liu, Zhiqiang
Qie, Shuang
Li, Lili
Xiu, Bingshui
Yang, Xiqin
Dai, Zhenhua
Zhang, Xuhui
Duan, Cuimi
Que, Haiping
Zhao, Ping
Johnson, Heather
Zhang, Heqiu
Feng, Xiaoyan
spellingShingle Liu, Zhiqiang
Qie, Shuang
Li, Lili
Xiu, Bingshui
Yang, Xiqin
Dai, Zhenhua
Zhang, Xuhui
Duan, Cuimi
Que, Haiping
Zhao, Ping
Johnson, Heather
Zhang, Heqiu
Feng, Xiaoyan
Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients
author_facet Liu, Zhiqiang
Qie, Shuang
Li, Lili
Xiu, Bingshui
Yang, Xiqin
Dai, Zhenhua
Zhang, Xuhui
Duan, Cuimi
Que, Haiping
Zhao, Ping
Johnson, Heather
Zhang, Heqiu
Feng, Xiaoyan
author_sort Liu, Zhiqiang
title Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients
title_short Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients
title_full Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients
title_fullStr Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients
title_full_unstemmed Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients
title_sort identification of novel rd1 antigens and their combinations for diagnosis of sputum smear−/culture+ tb patients
description Rapid and accurate diagnosis of pulmonary tuberculosis (PTB) is an unresolved problem worldwide, especially for sputum smear− (S−) cases. In this study, five antigen genes including Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 were cloned from Mycobacterium tuberculosis (Mtb) RD1 and overexpressed to generate antigen fragments. These antigens and their combinations were investigated for PTB serodiagnosis. 298 serum samples were collected from active PTB patients, including 117 sputum smear+ (S+) and sputum culture+ (C+) cases, 101 S−/C+ cases, and 80 S−/C− cases. The serum IgG levels of the five antigens were measured by ELISA. Based on IgG levels, the sensitivity/specificity of Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 for PTB detection was 81.21%/74.74%, 63.09%/94.78%, 32.21%/87.37%, 62.42%/85.26%, and 83.56%/83.16%, respectively. Furthermore, the optimal result for PTB diagnosis was achieved by combining antigens Rv3871, Rv3876, and Rv3879. In addition, the IgG levels of Rv3871, Rv3876, and Rv3879 were found to be higher in S−/C+ PTB patients than in other PTB populations. More importantly, combination of the three antigens demonstrated superior diagnostic performance for both S−/C+ and S−/C− PTB. In conclusion, the combination of Rv3871, Rv3876, and Rv3879 induced higher IgG response in sputum S−/C+ PTB patients and represents a promising biomarker combination for diagnosing of PTB.
publisher Hindawi Publishing Corporation
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4739478/
_version_ 1613533127254212608

Similar Items