MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum

Myeloid differentiation primary response gene 88 (MYD88), a universal adapter protein, plays an important role in activating the nuclear factor-κB (NF-κB) and regulating the expression of proinflammatory genes like tumor necrosis factor (TNF) and interleukin-1 (IL-1), which were highly involved in S...

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Main Authors:	Liu, Xian-Qing, Wang, Fei, Jin, Jie, Zhou, Yu-Guang, Ran, Jin-Shan, Feng, Ze-Qing, Wang, Yan, Liu, Yi-Ping
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735975/

id	pubmed-4735975
recordtype	oai_dc
spelling	pubmed-47359752016-02-15 MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum Liu, Xian-Qing Wang, Fei Jin, Jie Zhou, Yu-Guang Ran, Jin-Shan Feng, Ze-Qing Wang, Yan Liu, Yi-Ping Research Article Myeloid differentiation primary response gene 88 (MYD88), a universal adapter protein, plays an important role in activating the nuclear factor-κB (NF-κB) and regulating the expression of proinflammatory genes like tumor necrosis factor (TNF) and interleukin-1 (IL-1), which were highly involved in Salmonella Pullorum infection. To detect the relationship between polymorphisms of the MyD88 gene and Salmonella Pullorum disease, we screened the coding region (CDS) of the MYD88 gene by DNA pool construction and sequencing based on case-control study. Eight single nucleotide polymorphisms (SNPs) in the sequenced fragment (5 exons), 7 known loci and one novel mutation named G4810372T (SNP8), were found in the fifth exon. In addition, we found 7 nonsynonymous substitutions. The allele frequency of only one SNP, g.4810191C > T (SNP1), was significantly different (P < 0.05) between case and control groups. The genotype frequencies of SNP1 (g.4810191C > T) and SNP3 (g.4810257G > T) were of significant difference between the case and the control groups (P < 0.05). Collectively, SNPs of the MyD88 gene were significantly associated with susceptibility to Salmonella Pullorum infection, which can be used as a disease-resistant marker in chicken. These results provided a theoretical basis for future research on chicken breeding by marker-assisted selection. Hindawi Publishing Corporation 2015 2015-12-31 /pmc/articles/PMC4735975/ /pubmed/26881204 http://dx.doi.org/10.1155/2015/692973 Text en Copyright © 2015 Xian-Qing Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Liu, Xian-Qing Wang, Fei Jin, Jie Zhou, Yu-Guang Ran, Jin-Shan Feng, Ze-Qing Wang, Yan Liu, Yi-Ping
spellingShingle	Liu, Xian-Qing Wang, Fei Jin, Jie Zhou, Yu-Guang Ran, Jin-Shan Feng, Ze-Qing Wang, Yan Liu, Yi-Ping MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum
author_facet	Liu, Xian-Qing Wang, Fei Jin, Jie Zhou, Yu-Guang Ran, Jin-Shan Feng, Ze-Qing Wang, Yan Liu, Yi-Ping
author_sort	Liu, Xian-Qing
title	MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum
title_short	MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum
title_full	MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum
title_fullStr	MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum
title_full_unstemmed	MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum
title_sort	myd88 polymorphisms and association with susceptibility to salmonella pullorum
description	Myeloid differentiation primary response gene 88 (MYD88), a universal adapter protein, plays an important role in activating the nuclear factor-κB (NF-κB) and regulating the expression of proinflammatory genes like tumor necrosis factor (TNF) and interleukin-1 (IL-1), which were highly involved in Salmonella Pullorum infection. To detect the relationship between polymorphisms of the MyD88 gene and Salmonella Pullorum disease, we screened the coding region (CDS) of the MYD88 gene by DNA pool construction and sequencing based on case-control study. Eight single nucleotide polymorphisms (SNPs) in the sequenced fragment (5 exons), 7 known loci and one novel mutation named G4810372T (SNP8), were found in the fifth exon. In addition, we found 7 nonsynonymous substitutions. The allele frequency of only one SNP, g.4810191C > T (SNP1), was significantly different (P < 0.05) between case and control groups. The genotype frequencies of SNP1 (g.4810191C > T) and SNP3 (g.4810257G > T) were of significant difference between the case and the control groups (P < 0.05). Collectively, SNPs of the MyD88 gene were significantly associated with susceptibility to Salmonella Pullorum infection, which can be used as a disease-resistant marker in chicken. These results provided a theoretical basis for future research on chicken breeding by marker-assisted selection.
publisher	Hindawi Publishing Corporation
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735975/
_version_	1613531675552120832

MyD88 Polymorphisms and Association with Susceptibility to Salmonella Pullorum

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