An integrated multi-electrode-optrode array for in vitro optogenetics
Modulation of a group of cells or tissue needs to be very precise in order to exercise effective control over the cell population under investigation. Optogenetic tools have already demonstrated to be of great value in the study of neuronal circuits and in neuromodulation. Ideally, they should permi...
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| Format: | Online |
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735812/ |
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pubmed-47358122016-02-05 An integrated multi-electrode-optrode array for in vitro optogenetics Welkenhuysen, Marleen Hoffman, Luis Luo, Zhengxiang De Proft, Anabel Van den Haute, Chris Baekelandt, Veerle Debyser, Zeger Gielen, Georges Puers, Robert Braeken, Dries Article Modulation of a group of cells or tissue needs to be very precise in order to exercise effective control over the cell population under investigation. Optogenetic tools have already demonstrated to be of great value in the study of neuronal circuits and in neuromodulation. Ideally, they should permit very accurate resolution, preferably down to the single cell level. Further, to address a spatially distributed sample, independently addressable multiple optical outputs should be present. In current techniques, at least one of these requirements is not fulfilled. In addition to this, it is interesting to directly monitor feedback of the modulation by electrical registration of the activity of the stimulated cells. Here, we present the fabrication and characterization of a fully integrated silicon-based multi-electrode-optrode array (MEOA) for in vitro optogenetics. We demonstrate that this device allows for artifact-free electrical recording. Moreover, the MEOA was used to reliably elicit spiking activity from ChR2-transduced neurons. Thanks to the single cell resolution stimulation capability, we could determine spatial and temporal activation patterns and spike latencies of the neuronal network. This integrated approach to multi-site combined optical stimulation and electrical recording significantly advances today’s tool set for neuroscientists in their search to unravel neuronal network dynamics. Nature Publishing Group 2016-02-02 /pmc/articles/PMC4735812/ /pubmed/26832455 http://dx.doi.org/10.1038/srep20353 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Welkenhuysen, Marleen Hoffman, Luis Luo, Zhengxiang De Proft, Anabel Van den Haute, Chris Baekelandt, Veerle Debyser, Zeger Gielen, Georges Puers, Robert Braeken, Dries |
| spellingShingle |
Welkenhuysen, Marleen Hoffman, Luis Luo, Zhengxiang De Proft, Anabel Van den Haute, Chris Baekelandt, Veerle Debyser, Zeger Gielen, Georges Puers, Robert Braeken, Dries An integrated multi-electrode-optrode array for in vitro optogenetics |
| author_facet |
Welkenhuysen, Marleen Hoffman, Luis Luo, Zhengxiang De Proft, Anabel Van den Haute, Chris Baekelandt, Veerle Debyser, Zeger Gielen, Georges Puers, Robert Braeken, Dries |
| author_sort |
Welkenhuysen, Marleen |
| title |
An integrated multi-electrode-optrode array for in vitro optogenetics |
| title_short |
An integrated multi-electrode-optrode array for in vitro optogenetics |
| title_full |
An integrated multi-electrode-optrode array for in vitro optogenetics |
| title_fullStr |
An integrated multi-electrode-optrode array for in vitro optogenetics |
| title_full_unstemmed |
An integrated multi-electrode-optrode array for in vitro optogenetics |
| title_sort |
integrated multi-electrode-optrode array for in vitro optogenetics |
| description |
Modulation of a group of cells or tissue needs to be very precise in order to exercise effective control over the cell population under investigation. Optogenetic tools have already demonstrated to be of great value in the study of neuronal circuits and in neuromodulation. Ideally, they should permit very accurate resolution, preferably down to the single cell level. Further, to address a spatially distributed sample, independently addressable multiple optical outputs should be present. In current techniques, at least one of these requirements is not fulfilled. In addition to this, it is interesting to directly monitor feedback of the modulation by electrical registration of the activity of the stimulated cells. Here, we present the fabrication and characterization of a fully integrated silicon-based multi-electrode-optrode array (MEOA) for in vitro optogenetics. We demonstrate that this device allows for artifact-free electrical recording. Moreover, the MEOA was used to reliably elicit spiking activity from ChR2-transduced neurons. Thanks to the single cell resolution stimulation capability, we could determine spatial and temporal activation patterns and spike latencies of the neuronal network. This integrated approach to multi-site combined optical stimulation and electrical recording significantly advances today’s tool set for neuroscientists in their search to unravel neuronal network dynamics. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735812/ |
| _version_ |
1613531595761778688 |