Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border
The artemisinin (ART), discovered in China, has been widely used against malaria in China over the last 30 years. Understanding the emergence and origin of ART resistance in China is therefore of great interest. We analyzed 111 culture-adapted isolates of P. falciparum from China-Myanmar (CM) border...
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Nature Publishing Group
2016
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735722/ |
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pubmed-47357222016-02-05 Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border Ye, Run Hu, Dongwei Zhang, Yilong Huang, Yufu Sun, Xiaodong Wang, Jian Chen, Xuedi Zhou, Hongning Zhang, Dongmei Mungthin, Mathirut Pan, Weiqing Article The artemisinin (ART), discovered in China, has been widely used against malaria in China over the last 30 years. Understanding the emergence and origin of ART resistance in China is therefore of great interest. We analyzed 111 culture-adapted isolates of P. falciparum from China-Myanmar (CM) border for their susceptibility to dihydroartemisinin using the ring stage survival assay (RSA0−3h) and genotyped their K13 genes. Of the isolates, 59 had a wild type of the K13 marker and a median ring survival rate of 0.26% (P95 = 1.005%). Among the remaining isolates harboring single mutations in the K13 marker, 26 survived at >P95(median survival rate = 2.95%). Further, we genotyped the K13 gene of 693 isolates collected from different regions in China and China-Myanmar/Thai-Cambodia/Thai-Myanmar (CM/TC/TM) borders, 308 (44.4%) had K13 mutations and marked differences in the patterns of K13 mutations were observed between the CM and the TC/TM borders. A network diagram showed that majority of the K13 mutant alleles from the CM border clustered together including those harboring the high resistant-associated R539T mutations. The resistant parasites carrying distinct halplotypes suggested the multiple indigenous origins of the resistant alleles, which highlight the importance of surveillance of resistance in all malaria endemic areas where ART has been introduced. Nature Publishing Group 2016-02-02 /pmc/articles/PMC4735722/ /pubmed/26831371 http://dx.doi.org/10.1038/srep20100 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ye, Run Hu, Dongwei Zhang, Yilong Huang, Yufu Sun, Xiaodong Wang, Jian Chen, Xuedi Zhou, Hongning Zhang, Dongmei Mungthin, Mathirut Pan, Weiqing |
| spellingShingle |
Ye, Run Hu, Dongwei Zhang, Yilong Huang, Yufu Sun, Xiaodong Wang, Jian Chen, Xuedi Zhou, Hongning Zhang, Dongmei Mungthin, Mathirut Pan, Weiqing Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border |
| author_facet |
Ye, Run Hu, Dongwei Zhang, Yilong Huang, Yufu Sun, Xiaodong Wang, Jian Chen, Xuedi Zhou, Hongning Zhang, Dongmei Mungthin, Mathirut Pan, Weiqing |
| author_sort |
Ye, Run |
| title |
Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border |
| title_short |
Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border |
| title_full |
Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border |
| title_fullStr |
Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border |
| title_full_unstemmed |
Distinctive origin of artemisinin-resistant Plasmodium falciparum on the China-Myanmar border |
| title_sort |
distinctive origin of artemisinin-resistant plasmodium falciparum on the china-myanmar border |
| description |
The artemisinin (ART), discovered in China, has been widely used against malaria in China over the last 30 years. Understanding the emergence and origin of ART resistance in China is therefore of great interest. We analyzed 111 culture-adapted isolates of P. falciparum from China-Myanmar (CM) border for their susceptibility to dihydroartemisinin using the ring stage survival assay (RSA0−3h) and genotyped their K13 genes. Of the isolates, 59 had a wild type of the K13 marker and a median ring survival rate of 0.26% (P95 = 1.005%). Among the remaining isolates harboring single mutations in the K13 marker, 26 survived at >P95(median survival rate = 2.95%). Further, we genotyped the K13 gene of 693 isolates collected from different regions in China and China-Myanmar/Thai-Cambodia/Thai-Myanmar (CM/TC/TM) borders, 308 (44.4%) had K13 mutations and marked differences in the patterns of K13 mutations were observed between the CM and the TC/TM borders. A network diagram showed that majority of the K13 mutant alleles from the CM border clustered together including those harboring the high resistant-associated R539T mutations. The resistant parasites carrying distinct halplotypes suggested the multiple indigenous origins of the resistant alleles, which highlight the importance of surveillance of resistance in all malaria endemic areas where ART has been introduced. |
| publisher |
Nature Publishing Group |
| publishDate |
2016 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4735722/ |
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1613531549100146688 |