Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine

Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine

A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the construction of a label-free G-quadruplex-based...

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Main Authors: Lu, Lihua, Zhong, Hai-Jing, He, Bingyong, Leung, Chung-Hang, Ma, Dik-Lung
Format: Online
Language:English
Published: Nature Publishing Group 2016
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726048/
id pubmed-4726048
recordtype oai_dc
spelling pubmed-47260482016-01-28 Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine Lu, Lihua Zhong, Hai-Jing He, Bingyong Leung, Chung-Hang Ma, Dik-Lung Article A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection, and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R2 = 0.992), and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides, and exhibited potential use for biological sample analysis. Nature Publishing Group 2016-01-18 /pmc/articles/PMC4726048/ /pubmed/26778273 http://dx.doi.org/10.1038/srep19368 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Lu, Lihua
Zhong, Hai-Jing
He, Bingyong
Leung, Chung-Hang
Ma, Dik-Lung
spellingShingle Lu, Lihua
Zhong, Hai-Jing
He, Bingyong
Leung, Chung-Hang
Ma, Dik-Lung
Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
author_facet Lu, Lihua
Zhong, Hai-Jing
He, Bingyong
Leung, Chung-Hang
Ma, Dik-Lung
author_sort Lu, Lihua
title Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
title_short Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
title_full Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
title_fullStr Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
title_full_unstemmed Development of a luminescent G-quadruplex-selective iridium(III) complex for the label-free detection of adenosine
title_sort development of a luminescent g-quadruplex-selective iridium(iii) complex for the label-free detection of adenosine
description A panel of six luminescent iridium(III) complexes were synthesized and evaluated for their ability to act as G-quadruplex-selective probes. The novel iridium(III) complex 1 was found to be highly selective for G-quadruplex DNA, and was employed for the construction of a label-free G-quadruplex-based adenosine detection assay in aqueous solution. Two different detection strategies were investigated for adenosine detection, and the results showed that initial addition of adenosine to the adenosine aptamer gave superior results. The assay exhibited a linear response for adenosine in the concentration range of 5 to 120 μM (R2 = 0.992), and the limit of detection for adenosine was 5 μM. Moreover, this assay was highly selective for adenosine over other nucleosides, and exhibited potential use for biological sample analysis.
publisher Nature Publishing Group
publishDate 2016
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726048/
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