Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure

Thirteen morphologically similar strains of barbatosphaeria- and tectonidula-like fungi were studied based on the comparison of cultural and morphological features of sexual and asexual morphs and phylogenetic analyses of five nuclear loci, i.e. internal transcribed spacer rDNA operon (ITS), large a...

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Main Authors:	Réblová, M., Réblová, K., Štěpánek, V.
Format:	Online
Language:	English
Published:	Naturalis Biodiversity Center & Centraallbureau voor Schimmelcultures 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713105/

id	pubmed-4713105
recordtype	oai_dc
spelling	pubmed-47131052016-01-28 Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure Réblová, M. Réblová, K. Štěpánek, V. Research Article Thirteen morphologically similar strains of barbatosphaeria- and tectonidula-like fungi were studied based on the comparison of cultural and morphological features of sexual and asexual morphs and phylogenetic analyses of five nuclear loci, i.e. internal transcribed spacer rDNA operon (ITS), large and small subunit nuclear ribosomal DNA, β-tubulin, and second largest subunit of RNA polymerase II. Phylogenetic results were supported by in-depth comparative analyses of common core secondary structure of ITS1 and ITS2 in all strains and the identification of non-conserved, co-evolving nucleotides that maintain base pairing in the RNA transcript. Barbatosphaeria is defined as a well-supported monophyletic clade comprising several lineages and is placed in the Sordariomycetes incertae sedis. The genus is expanded to encompass nine species with both septate and non-septate ascospores in clavate, stipitate asci with a non-amyloid apical annulus and non-stromatic ascomata with a long decumbent neck and carbonised wall often covered by pubescence. The asexual morphs are dematiaceous hyphomycetes with holoblastic conidiogenesis belonging to Ramichloridium and Sporothrix types. The morphologically similar Tectonidula, represented by the type species T. hippocrepida, grouped with members of Barbatosphaeria and is transferred to that genus. Four new species are introduced and three new combinations in Barbatosphaeria are proposed. A dichotomous key to species accepted in the genus is provided. Naturalis Biodiversity Center & Centraallbureau voor Schimmelcultures 2015-02-02 2015-12 /pmc/articles/PMC4713105/ /pubmed/26823626 http://dx.doi.org/10.3767/003158515X687434 Text en © 2015 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Réblová, M. Réblová, K. Štěpánek, V.
spellingShingle	Réblová, M. Réblová, K. Štěpánek, V. Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure
author_facet	Réblová, M. Réblová, K. Štěpánek, V.
author_sort	Réblová, M.
title	Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure
title_short	Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure
title_full	Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure
title_fullStr	Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure
title_full_unstemmed	Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure
title_sort	molecular systematics of barbatosphaeria (sordariomycetes): multigene phylogeny and secondary its structure
description	Thirteen morphologically similar strains of barbatosphaeria- and tectonidula-like fungi were studied based on the comparison of cultural and morphological features of sexual and asexual morphs and phylogenetic analyses of five nuclear loci, i.e. internal transcribed spacer rDNA operon (ITS), large and small subunit nuclear ribosomal DNA, β-tubulin, and second largest subunit of RNA polymerase II. Phylogenetic results were supported by in-depth comparative analyses of common core secondary structure of ITS1 and ITS2 in all strains and the identification of non-conserved, co-evolving nucleotides that maintain base pairing in the RNA transcript. Barbatosphaeria is defined as a well-supported monophyletic clade comprising several lineages and is placed in the Sordariomycetes incertae sedis. The genus is expanded to encompass nine species with both septate and non-septate ascospores in clavate, stipitate asci with a non-amyloid apical annulus and non-stromatic ascomata with a long decumbent neck and carbonised wall often covered by pubescence. The asexual morphs are dematiaceous hyphomycetes with holoblastic conidiogenesis belonging to Ramichloridium and Sporothrix types. The morphologically similar Tectonidula, represented by the type species T. hippocrepida, grouped with members of Barbatosphaeria and is transferred to that genus. Four new species are introduced and three new combinations in Barbatosphaeria are proposed. A dichotomous key to species accepted in the genus is provided.
publisher	Naturalis Biodiversity Center & Centraallbureau voor Schimmelcultures
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713105/
_version_	1613524099992125440

Molecular systematics of Barbatosphaeria (Sordariomycetes): multigene phylogeny and secondary ITS structure

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