Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses

Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses

Dietary phenolics may play a protective role in UV-mediated skin pigmentation through their antioxidant and UV-absorbing actions. In this study, we investigated whether genetic silencing of Nrf2, regulating the transcription of antioxidant genes, affected melanogenesis in primary human epidermal mel...

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Main Authors: Chaiprasongsuk, Anyamanee, Onkoksoong, Tasanee, Pluemsamran, Thanyawan, Limsaengurai, Saowalak, Panich, Uraiwan
Format: Online
Language:English
Published: Elsevier 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712325/
id pubmed-4712325
recordtype oai_dc
spelling pubmed-47123252016-02-11 Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses Chaiprasongsuk, Anyamanee Onkoksoong, Tasanee Pluemsamran, Thanyawan Limsaengurai, Saowalak Panich, Uraiwan Research Paper Dietary phenolics may play a protective role in UV-mediated skin pigmentation through their antioxidant and UV-absorbing actions. In this study, we investigated whether genetic silencing of Nrf2, regulating the transcription of antioxidant genes, affected melanogenesis in primary human epidermal melanocytes (HEMn) and B16F10 melanoma cells subjected to UVA (8 J/cm2) exposure. Then, we explored the antimelanogenic actions of phenolics; caffeic acid (CA) and ferulic acid (FA) providing partial UVA protection; quercetin (QU) and rutin (RU) providing strong UVA protection and; avobenzone (AV), an efficient UVA filter, in association with modulation of Nrf2-mediated antioxidant defenses in response to UVA insults in B16F10 cells. Upon oxidative insults, Nrf2 silencing promoted melanogenesis in both HEMn and B16F10 cells irradiated with UVA. Stimulation of melanogenesis by UVA correlated with increased ROS and oxidative DNA damage (8-OHdG), GSH depletion as well as a transient downregulation of Nrf2 nuclear translocation and of Nrf2-ARE signaling in B16F10 cells. All test compounds exerted antimelanogenic effects with respect to their abilities to reverse UVA-mediated oxidative damage as well as downregulation of Nrf2 activity and its target antioxidants (GCLC, GST and NQO1) in B16F10 cells. In conclusion, defective Nrf2 may promote melanogenesis under UVA irradiation through oxidative stress mechanisms. Compounds with antioxidant and/or UVA absorption properties could protect against UVA-induced melanogenesis through indirect regulatory effect on Nrf2-ARE pathway. Elsevier 2015-12-19 /pmc/articles/PMC4712325/ /pubmed/26765101 http://dx.doi.org/10.1016/j.redox.2015.12.006 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Chaiprasongsuk, Anyamanee
Onkoksoong, Tasanee
Pluemsamran, Thanyawan
Limsaengurai, Saowalak
Panich, Uraiwan
spellingShingle Chaiprasongsuk, Anyamanee
Onkoksoong, Tasanee
Pluemsamran, Thanyawan
Limsaengurai, Saowalak
Panich, Uraiwan
Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses
author_facet Chaiprasongsuk, Anyamanee
Onkoksoong, Tasanee
Pluemsamran, Thanyawan
Limsaengurai, Saowalak
Panich, Uraiwan
author_sort Chaiprasongsuk, Anyamanee
title Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses
title_short Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses
title_full Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses
title_fullStr Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses
title_full_unstemmed Photoprotection by dietary phenolics against melanogenesis induced by UVA through Nrf2-dependent antioxidant responses
title_sort photoprotection by dietary phenolics against melanogenesis induced by uva through nrf2-dependent antioxidant responses
description Dietary phenolics may play a protective role in UV-mediated skin pigmentation through their antioxidant and UV-absorbing actions. In this study, we investigated whether genetic silencing of Nrf2, regulating the transcription of antioxidant genes, affected melanogenesis in primary human epidermal melanocytes (HEMn) and B16F10 melanoma cells subjected to UVA (8 J/cm2) exposure. Then, we explored the antimelanogenic actions of phenolics; caffeic acid (CA) and ferulic acid (FA) providing partial UVA protection; quercetin (QU) and rutin (RU) providing strong UVA protection and; avobenzone (AV), an efficient UVA filter, in association with modulation of Nrf2-mediated antioxidant defenses in response to UVA insults in B16F10 cells. Upon oxidative insults, Nrf2 silencing promoted melanogenesis in both HEMn and B16F10 cells irradiated with UVA. Stimulation of melanogenesis by UVA correlated with increased ROS and oxidative DNA damage (8-OHdG), GSH depletion as well as a transient downregulation of Nrf2 nuclear translocation and of Nrf2-ARE signaling in B16F10 cells. All test compounds exerted antimelanogenic effects with respect to their abilities to reverse UVA-mediated oxidative damage as well as downregulation of Nrf2 activity and its target antioxidants (GCLC, GST and NQO1) in B16F10 cells. In conclusion, defective Nrf2 may promote melanogenesis under UVA irradiation through oxidative stress mechanisms. Compounds with antioxidant and/or UVA absorption properties could protect against UVA-induced melanogenesis through indirect regulatory effect on Nrf2-ARE pathway.
publisher Elsevier
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712325/
_version_ 1613523862952083456

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