Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging

In this study, the impact of future climate change on photosynthetic efficiency as well as energy partitioning in the Stipa bungeana was investigated by using chlorophyll fluorescence imaging (CFI) technique. Two thermal regimes (room temperature, T0: 23.0/17.0°C; High temperature, T6: 29.0/23.0°C)...

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Main Authors:	Song, Xiliang, Zhou, Guangsheng, Xu, Zhenzhu, Lv, Xiaomin, Wang, Yuhui
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709831/

id	pubmed-4709831
recordtype	oai_dc
spelling	pubmed-47098312016-01-20 Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging Song, Xiliang Zhou, Guangsheng Xu, Zhenzhu Lv, Xiaomin Wang, Yuhui Plant Science In this study, the impact of future climate change on photosynthetic efficiency as well as energy partitioning in the Stipa bungeana was investigated by using chlorophyll fluorescence imaging (CFI) technique. Two thermal regimes (room temperature, T0: 23.0/17.0°C; High temperature, T6: 29.0/23.0°C) and three water conditions (Control, W0; Water deficit, W−30; excess precipitation, W+30) were set up in artificial control chambers. The results showed that excess precipitation had no significant effect on chlorophyll fluorescence parameters, while water deficit decreased the maximal quantum yield of photosystem II (PSII) photochemistry for the dark-adapted state (Fv/Fm) by 16.7%, with no large change in maximal quantum yield of PSII photochemistry for the light-adapted state (FV′/FM′) and coefficient of the photochemical quenching (qP) at T0 condition. Under T6 condition, high temperature offset the negative effect of water deficit on Fv/Fm and enhanced the positive effect of excess precipitation on Fv/Fm, Fv′/Fm′, and qP, the values of which all increased. This indicates that the temperature higher by 6°C will be beneficial to the photosynthetic performance of S. bungeana. Spatial changes of photosynthetic performance were monitored in three areas of interest (AOIs) located on the bottom, middle and upper position of leaf. Chlorophyll fluorescence images (Fv/Fm, actual quantum yield of PSII photochemistry for the light-adapted state (ΦPSII), quantum yield of non-regulated energy dissipation for the light-adapted state (ΦNO) at T0 condition, and ΦPSII at T6 condition) showed a large spatial variation, with greater value of ΦNO and lower values of Fv/Fm and ΦPSII in the upper position of leaves. Moreover, there was a closer relationship between ΦPSII and ΦNO, suggesting that the energy dissipation by non-regulated quenching mechanisms played a dominant role in the yield of PSII photochemistry. It was also found that, among all measured fluorescence parameters, the Fv/Fm ratio was most sensitive to precipitation change at T0, while ΦPSII was the most sensitive indicator at T6. Frontiers Media S.A. 2016-01-12 /pmc/articles/PMC4709831/ /pubmed/26793224 http://dx.doi.org/10.3389/fpls.2015.01254 Text en Copyright © 2016 Song, Zhou, Xu, Lv and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Song, Xiliang Zhou, Guangsheng Xu, Zhenzhu Lv, Xiaomin Wang, Yuhui
spellingShingle	Song, Xiliang Zhou, Guangsheng Xu, Zhenzhu Lv, Xiaomin Wang, Yuhui Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging
author_facet	Song, Xiliang Zhou, Guangsheng Xu, Zhenzhu Lv, Xiaomin Wang, Yuhui
author_sort	Song, Xiliang
title	Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging
title_short	Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging
title_full	Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging
title_fullStr	Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging
title_full_unstemmed	Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging
title_sort	detection of photosynthetic performance of stipa bungeana seedlings under climatic change using chlorophyll fluorescence imaging
description	In this study, the impact of future climate change on photosynthetic efficiency as well as energy partitioning in the Stipa bungeana was investigated by using chlorophyll fluorescence imaging (CFI) technique. Two thermal regimes (room temperature, T0: 23.0/17.0°C; High temperature, T6: 29.0/23.0°C) and three water conditions (Control, W0; Water deficit, W−30; excess precipitation, W+30) were set up in artificial control chambers. The results showed that excess precipitation had no significant effect on chlorophyll fluorescence parameters, while water deficit decreased the maximal quantum yield of photosystem II (PSII) photochemistry for the dark-adapted state (Fv/Fm) by 16.7%, with no large change in maximal quantum yield of PSII photochemistry for the light-adapted state (FV′/FM′) and coefficient of the photochemical quenching (qP) at T0 condition. Under T6 condition, high temperature offset the negative effect of water deficit on Fv/Fm and enhanced the positive effect of excess precipitation on Fv/Fm, Fv′/Fm′, and qP, the values of which all increased. This indicates that the temperature higher by 6°C will be beneficial to the photosynthetic performance of S. bungeana. Spatial changes of photosynthetic performance were monitored in three areas of interest (AOIs) located on the bottom, middle and upper position of leaf. Chlorophyll fluorescence images (Fv/Fm, actual quantum yield of PSII photochemistry for the light-adapted state (ΦPSII), quantum yield of non-regulated energy dissipation for the light-adapted state (ΦNO) at T0 condition, and ΦPSII at T6 condition) showed a large spatial variation, with greater value of ΦNO and lower values of Fv/Fm and ΦPSII in the upper position of leaves. Moreover, there was a closer relationship between ΦPSII and ΦNO, suggesting that the energy dissipation by non-regulated quenching mechanisms played a dominant role in the yield of PSII photochemistry. It was also found that, among all measured fluorescence parameters, the Fv/Fm ratio was most sensitive to precipitation change at T0, while ΦPSII was the most sensitive indicator at T6.
publisher	Frontiers Media S.A.
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709831/
_version_	1613523062666297344

Detection of Photosynthetic Performance of Stipa bungeana Seedlings under Climatic Change using Chlorophyll Fluorescence Imaging

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