Evaluating the use of HILIC in large-scale, multi dimensional proteomics: Horses for courses?
•HILIC provides a robust and reproducible off-line LC method for 2D-LC deep proteomics workflows.•HILIC is part-orthogonal to hSAX fractionation.•HILIC-based workflow identified ∼81,000 peptides from >9000 proteins with an average sequence coverage of ∼26%.•HILIC is well suited for the study of h...
| Main Authors: | , , |
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| Format: | Online |
| Language: | English |
| Published: |
Elsevier
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708065/ |