Carbazole degradation in the soil microcosm by tropical bacterial strains

In a previous study, three bacterial strains isolated from tropical hydrocarbon-contaminated soils and phylogenetically identified as Achromobacter sp. strain SL1, Pseudomonas sp. strain SL4 and Microbacterium esteraromaticum strain SL6 displayed angular dioxygenation and mineralization of carbazole...

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Main Authors:	Salam, Lateef B., Ilori, Matthew O., Amund, Olukayode O.
Format:	Online
Language:	English
Published:	Sociedade Brasileira de Microbiologia 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704645/

id	pubmed-4704645
recordtype	oai_dc
spelling	pubmed-47046452016-01-14 Carbazole degradation in the soil microcosm by tropical bacterial strains Salam, Lateef B. Ilori, Matthew O. Amund, Olukayode O. Environmental Microbiology In a previous study, three bacterial strains isolated from tropical hydrocarbon-contaminated soils and phylogenetically identified as Achromobacter sp. strain SL1, Pseudomonas sp. strain SL4 and Microbacterium esteraromaticum strain SL6 displayed angular dioxygenation and mineralization of carbazole in batch cultures. In this study, the ability of these isolates to survive and enhance carbazole degradation in soil were tested in field-moist microcosms. Strain SL4 had the highest survival rate (1.8 x 107 cfu/g) after 30 days of incubation in sterilized soil, while there was a decrease in population density in native (unsterilized) soil when compared with the initial population. Gas chromatographic analysis after 30 days of incubation showed that in sterilized soil amended with carbazole (100 mg/kg), 66.96, 82.15 and 68.54% were degraded by strains SL1, SL4 and SL6, respectively, with rates of degradation of 0.093, 0.114 and 0.095 mg kg−1 h−1. The combination of the three isolates as inoculum in sterilized soil degraded 87.13% carbazole at a rate of 0.121 mg kg−1 h−1. In native soil amended with carbazole (100 mg/kg), 91.64, 87.29 and 89.13% were degraded by strains SL1, SL4 and SL6 after 30 days of incubation, with rates of degradation of 0.127, 0.121 and 0.124 mg kg−1 h−1, respectively. This study successfully established the survivability (> 106 cfu/g detected after 30 days) and carbazole-degrading ability of these bacterial strains in soil, and highlights the potential of these isolates as seed for the bioremediation of carbazole-impacted environments. Sociedade Brasileira de Microbiologia 2015-12-01 /pmc/articles/PMC4704645/ /pubmed/26691461 http://dx.doi.org/10.1590/S1517-838246420140610 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/4.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Salam, Lateef B. Ilori, Matthew O. Amund, Olukayode O.
spellingShingle	Salam, Lateef B. Ilori, Matthew O. Amund, Olukayode O. Carbazole degradation in the soil microcosm by tropical bacterial strains
author_facet	Salam, Lateef B. Ilori, Matthew O. Amund, Olukayode O.
author_sort	Salam, Lateef B.
title	Carbazole degradation in the soil microcosm by tropical bacterial strains
title_short	Carbazole degradation in the soil microcosm by tropical bacterial strains
title_full	Carbazole degradation in the soil microcosm by tropical bacterial strains
title_fullStr	Carbazole degradation in the soil microcosm by tropical bacterial strains
title_full_unstemmed	Carbazole degradation in the soil microcosm by tropical bacterial strains
title_sort	carbazole degradation in the soil microcosm by tropical bacterial strains
description	In a previous study, three bacterial strains isolated from tropical hydrocarbon-contaminated soils and phylogenetically identified as Achromobacter sp. strain SL1, Pseudomonas sp. strain SL4 and Microbacterium esteraromaticum strain SL6 displayed angular dioxygenation and mineralization of carbazole in batch cultures. In this study, the ability of these isolates to survive and enhance carbazole degradation in soil were tested in field-moist microcosms. Strain SL4 had the highest survival rate (1.8 x 107 cfu/g) after 30 days of incubation in sterilized soil, while there was a decrease in population density in native (unsterilized) soil when compared with the initial population. Gas chromatographic analysis after 30 days of incubation showed that in sterilized soil amended with carbazole (100 mg/kg), 66.96, 82.15 and 68.54% were degraded by strains SL1, SL4 and SL6, respectively, with rates of degradation of 0.093, 0.114 and 0.095 mg kg−1 h−1. The combination of the three isolates as inoculum in sterilized soil degraded 87.13% carbazole at a rate of 0.121 mg kg−1 h−1. In native soil amended with carbazole (100 mg/kg), 91.64, 87.29 and 89.13% were degraded by strains SL1, SL4 and SL6 after 30 days of incubation, with rates of degradation of 0.127, 0.121 and 0.124 mg kg−1 h−1, respectively. This study successfully established the survivability (> 106 cfu/g detected after 30 days) and carbazole-degrading ability of these bacterial strains in soil, and highlights the potential of these isolates as seed for the bioremediation of carbazole-impacted environments.
publisher	Sociedade Brasileira de Microbiologia
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4704645/
_version_	1613521037935247360

Carbazole degradation in the soil microcosm by tropical bacterial strains

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