OpenTein: a database of digital whole-slide images of stem cell-derived teratomas

Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells...

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Main Authors:	Park, Sung-Joon, Komiyama, Yusuke, Suemori, Hirofumi, Umezawa, Akihiro, Nakai, Kenta
Format:	Online
Language:	English
Published:	Oxford University Press 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702800/

id	pubmed-4702800
recordtype	oai_dc
spelling	pubmed-47028002016-01-07 OpenTein: a database of digital whole-slide images of stem cell-derived teratomas Park, Sung-Joon Komiyama, Yusuke Suemori, Hirofumi Umezawa, Akihiro Nakai, Kenta Database Issue Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three germ layers, and the teratomas can be inspected through high-quality digital images. The teratoma assay, however, lacks consistency in transplantation protocols and even in interpretation, which needs community-based efforts for improving the assay quality. Here, we have developed a novel database OpenTein (Open Teratoma Investigation, http://opentein.hgc.jp/) to archive and freely distribute high-resolution whole-slide images and relevant records. OpenTein has been designed as a searchable, zoomable and annotatable web-based repository system. We have deposited 468 images of teratomas derived by our transplantation of human stem cells, and users can freely access and process such digital teratoma images. Approximately, the current version of OpenTein responds within 11.2 min for processing 2.03 gigapixel teratoma images. Our system offers valuable tools and resources in the new era of stem cell biology. Oxford University Press 2016-01-04 2015-10-22 /pmc/articles/PMC4702800/ /pubmed/26496950 http://dx.doi.org/10.1093/nar/gkv1096 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Park, Sung-Joon Komiyama, Yusuke Suemori, Hirofumi Umezawa, Akihiro Nakai, Kenta
spellingShingle	Park, Sung-Joon Komiyama, Yusuke Suemori, Hirofumi Umezawa, Akihiro Nakai, Kenta OpenTein: a database of digital whole-slide images of stem cell-derived teratomas
author_facet	Park, Sung-Joon Komiyama, Yusuke Suemori, Hirofumi Umezawa, Akihiro Nakai, Kenta
author_sort	Park, Sung-Joon
title	OpenTein: a database of digital whole-slide images of stem cell-derived teratomas
title_short	OpenTein: a database of digital whole-slide images of stem cell-derived teratomas
title_full	OpenTein: a database of digital whole-slide images of stem cell-derived teratomas
title_fullStr	OpenTein: a database of digital whole-slide images of stem cell-derived teratomas
title_full_unstemmed	OpenTein: a database of digital whole-slide images of stem cell-derived teratomas
title_sort	opentein: a database of digital whole-slide images of stem cell-derived teratomas
description	Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three germ layers, and the teratomas can be inspected through high-quality digital images. The teratoma assay, however, lacks consistency in transplantation protocols and even in interpretation, which needs community-based efforts for improving the assay quality. Here, we have developed a novel database OpenTein (Open Teratoma Investigation, http://opentein.hgc.jp/) to archive and freely distribute high-resolution whole-slide images and relevant records. OpenTein has been designed as a searchable, zoomable and annotatable web-based repository system. We have deposited 468 images of teratomas derived by our transplantation of human stem cells, and users can freely access and process such digital teratoma images. Approximately, the current version of OpenTein responds within 11.2 min for processing 2.03 gigapixel teratoma images. Our system offers valuable tools and resources in the new era of stem cell biology.
publisher	Oxford University Press
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4702800/
_version_	1613520463488614400

OpenTein: a database of digital whole-slide images of stem cell-derived teratomas

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