Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors

Histamine is an important chemical messenger that regulates multiple physiological processes in both vertebrate and invertebrate animals. Even so, how glial cells and neurons recycle histamine remains to be elucidated. Drosophila photoreceptor neurons use histamine as a neurotransmitter, and the rel...

Full description

Bibliographic Details
Main Authors:	Xu, Ying, An, Futing, Borycz, Jolanta A., Borycz, Janusz, Meinertzhagen, Ian A., Wang, Tao
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694695/

id	pubmed-4694695
recordtype	oai_dc
spelling	pubmed-46946952016-01-13 Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors Xu, Ying An, Futing Borycz, Jolanta A. Borycz, Janusz Meinertzhagen, Ian A. Wang, Tao Research Article Histamine is an important chemical messenger that regulates multiple physiological processes in both vertebrate and invertebrate animals. Even so, how glial cells and neurons recycle histamine remains to be elucidated. Drosophila photoreceptor neurons use histamine as a neurotransmitter, and the released histamine is recycled through neighboring glia, where it is conjugated to β-alanine to form carcinine. However, how carcinine is then returned to the photoreceptor remains unclear. In an mRNA-seq screen for photoreceptor cell-enriched transporters, we identified CG9317, an SLC22 transporter family protein, and named it CarT (Carcinine Transporter). S2 cells that express CarT are able to take up carcinine in vitro. In the compound eye, CarT is exclusively localized to photoreceptor terminals. Null mutations of cart alter the content of histamine and its metabolites. Moreover, null cart mutants are defective in photoreceptor synaptic transmission and lack phototaxis. These findings reveal that CarT is required for histamine recycling at histaminergic photoreceptors and provide evidence for a CarT-dependent neurotransmitter trafficking pathway between glial cells and photoreceptor terminals. Public Library of Science 2015-12-29 /pmc/articles/PMC4694695/ /pubmed/26713872 http://dx.doi.org/10.1371/journal.pgen.1005764 Text en © 2015 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Xu, Ying An, Futing Borycz, Jolanta A. Borycz, Janusz Meinertzhagen, Ian A. Wang, Tao
spellingShingle	Xu, Ying An, Futing Borycz, Jolanta A. Borycz, Janusz Meinertzhagen, Ian A. Wang, Tao Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
author_facet	Xu, Ying An, Futing Borycz, Jolanta A. Borycz, Janusz Meinertzhagen, Ian A. Wang, Tao
author_sort	Xu, Ying
title	Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
title_short	Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
title_full	Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
title_fullStr	Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
title_full_unstemmed	Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors
title_sort	histamine recycling is mediated by cart, a carcinine transporter in drosophila photoreceptors
description	Histamine is an important chemical messenger that regulates multiple physiological processes in both vertebrate and invertebrate animals. Even so, how glial cells and neurons recycle histamine remains to be elucidated. Drosophila photoreceptor neurons use histamine as a neurotransmitter, and the released histamine is recycled through neighboring glia, where it is conjugated to β-alanine to form carcinine. However, how carcinine is then returned to the photoreceptor remains unclear. In an mRNA-seq screen for photoreceptor cell-enriched transporters, we identified CG9317, an SLC22 transporter family protein, and named it CarT (Carcinine Transporter). S2 cells that express CarT are able to take up carcinine in vitro. In the compound eye, CarT is exclusively localized to photoreceptor terminals. Null mutations of cart alter the content of histamine and its metabolites. Moreover, null cart mutants are defective in photoreceptor synaptic transmission and lack phototaxis. These findings reveal that CarT is required for histamine recycling at histaminergic photoreceptors and provide evidence for a CarT-dependent neurotransmitter trafficking pathway between glial cells and photoreceptor terminals.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694695/
_version_	1613517701500633088

Histamine Recycling Is Mediated by CarT, a Carcinine Transporter in Drosophila Photoreceptors

Similar Items