Integration of an [FeFe]-hydrogenase into the anaerobic metabolism of Escherichia coli
•Engineering microbial biohydrogen production may have biotechnological applications.•A synthetic operon encoding an NADH-linked [FeFe]-hydrogenase was designed.•The enzyme was heterologously produced, activated and characterised.•The addition of ferredoxin and pyruvate oxidoreductase was necessary...
| Main Authors: | , , , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Elsevier
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694547/ |