Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum

Ralstonia solanacearum, the causal agent of bacterial wilt, can naturally infect a wide range of host plants. The type III secretion system (T3SS) is a major virulence determinant in this bacterium. Studies have shown that plant-derived compounds are able to inhibit or induce the T3SS in some plant...

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Main Authors:	Wu, Dousheng, Ding, Wei, Zhang, Yong, Liu, Xuejiao, Yang, Liang
Format:	Online
Language:	English
Published:	Frontiers Media S.A. 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686671/

id	pubmed-4686671
recordtype	oai_dc
spelling	pubmed-46866712016-01-05 Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum Wu, Dousheng Ding, Wei Zhang, Yong Liu, Xuejiao Yang, Liang Microbiology Ralstonia solanacearum, the causal agent of bacterial wilt, can naturally infect a wide range of host plants. The type III secretion system (T3SS) is a major virulence determinant in this bacterium. Studies have shown that plant-derived compounds are able to inhibit or induce the T3SS in some plant pathogenic bacteria, though no specific T3SS inhibitor or inducer has yet been identified in R. solanacearum. In this study, a total of 50 different compounds were screened and almost half of them (22 of 50) significantly inhibited or induced the T3SS expression of R. solanacearum. Based on the strong induction activity on T3SS, the T3SS inducer oleanolic acid (OA) was chosen for further study. We found that OA induced the expression of T3SS through the HrpG-HrpB pathway. Some type III effector genes were induced in T3SS inducing medium supplemented with OA. In addition, OA targeted only the T3SS and did not affect other virulence determinants. Finally, we observed that induction of T3SS by OA accelerated disease progress on tobacco. Overall our results suggest that plant-derived compounds are an abundant source of R. solanacearum T3SS regulators, which could prove useful as tools to interrogate the regulation of this key virulence pathway. Frontiers Media S.A. 2015-12-22 /pmc/articles/PMC4686671/ /pubmed/26732647 http://dx.doi.org/10.3389/fmicb.2015.01466 Text en Copyright © 2015 Wu, Ding, Zhang, Liu and Yang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wu, Dousheng Ding, Wei Zhang, Yong Liu, Xuejiao Yang, Liang
spellingShingle	Wu, Dousheng Ding, Wei Zhang, Yong Liu, Xuejiao Yang, Liang Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum
author_facet	Wu, Dousheng Ding, Wei Zhang, Yong Liu, Xuejiao Yang, Liang
author_sort	Wu, Dousheng
title	Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum
title_short	Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum
title_full	Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum
title_fullStr	Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum
title_full_unstemmed	Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum
title_sort	oleanolic acid induces the type iii secretion system of ralstonia solanacearum
description	Ralstonia solanacearum, the causal agent of bacterial wilt, can naturally infect a wide range of host plants. The type III secretion system (T3SS) is a major virulence determinant in this bacterium. Studies have shown that plant-derived compounds are able to inhibit or induce the T3SS in some plant pathogenic bacteria, though no specific T3SS inhibitor or inducer has yet been identified in R. solanacearum. In this study, a total of 50 different compounds were screened and almost half of them (22 of 50) significantly inhibited or induced the T3SS expression of R. solanacearum. Based on the strong induction activity on T3SS, the T3SS inducer oleanolic acid (OA) was chosen for further study. We found that OA induced the expression of T3SS through the HrpG-HrpB pathway. Some type III effector genes were induced in T3SS inducing medium supplemented with OA. In addition, OA targeted only the T3SS and did not affect other virulence determinants. Finally, we observed that induction of T3SS by OA accelerated disease progress on tobacco. Overall our results suggest that plant-derived compounds are an abundant source of R. solanacearum T3SS regulators, which could prove useful as tools to interrogate the regulation of this key virulence pathway.
publisher	Frontiers Media S.A.
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686671/
_version_	1613515246895366144

Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum

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