Identification of the nucleophile catalytic residue of GH51 α-l-arabinofuranosidase from Pleurotus ostreatus
In this study, the recombinant α-l-arabinofuranosidase from the fungus Pleurotus ostreatus (rPoAbf) was subjected to site-directed mutagenesis in order to identify the catalytic nucleophile residue. Based on bioinformatics and homology modelling analyses, E449 was revealed to be the potential nucleo...
| Main Authors: | , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Springer Berlin Heidelberg
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4686458/ |