Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood

Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was di...

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Main Authors:	Chen, Gecai, Yue, Aihuan, Ruan, Zhongbao, Yin, Yigang, Wang, Ruzhu, Ren, Yin, Zhu, Li
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2016
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685149/

id	pubmed-4685149
recordtype	oai_dc
spelling	pubmed-46851492016-01-14 Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood Chen, Gecai Yue, Aihuan Ruan, Zhongbao Yin, Yigang Wang, Ruzhu Ren, Yin Zhu, Li Research Article Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34+, cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34+ count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P < 0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome. Hindawi Publishing Corporation 2016 2015-12-07 /pmc/articles/PMC4685149/ /pubmed/26770201 http://dx.doi.org/10.1155/2016/1396783 Text en Copyright © 2016 Gecai Chen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Chen, Gecai Yue, Aihuan Ruan, Zhongbao Yin, Yigang Wang, Ruzhu Ren, Yin Zhu, Li
spellingShingle	Chen, Gecai Yue, Aihuan Ruan, Zhongbao Yin, Yigang Wang, Ruzhu Ren, Yin Zhu, Li Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
author_facet	Chen, Gecai Yue, Aihuan Ruan, Zhongbao Yin, Yigang Wang, Ruzhu Ren, Yin Zhu, Li
author_sort	Chen, Gecai
title	Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
title_short	Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
title_full	Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
title_fullStr	Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
title_full_unstemmed	Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood
title_sort	comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood
description	Purpose. Cryoprotectants (CPA) for stem cells from umbilical cord blood (UCB) have been widely developed based on empirical evidence, but there is no consensus on a standard protocol of preservation of the UCB cells. Methods. In this study, UCB from 115 donors was collected. Each unit of UCB was divided into four equal parts and frozen in different kinds of cryoprotectant as follows: group A, 10% ethylene glycol and 2.0% dimethyl sulfoxide (DMSO) (v/v); group B, 10% DMSO and 2.0% dextran-40; group C, 2.5% DMSO (v/v) + 30 mmol/L trehalose; and group D, without CPA. Results. CD34+, cell viability, colony forming units (CFUs), and cell apoptosis of pre- and postcryopreservation using three cryoprotectants were analyzed. After thawing, significant differences in CD34+ count, CFUs, cell apoptosis, and cell viability were observed among the four groups (P < 0.05). Conclusion. The low concentration of DMSO with the addition of trehalose might improve the cryopreservation outcome.
publisher	Hindawi Publishing Corporation
publishDate	2016
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685149/
_version_	1613514733774700544

Comparison of the Effects of Different Cryoprotectants on Stem Cells from Umbilical Cord Blood

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