Surgical Modification of the Murine Calvaria Osteolysis Model
The murine calvaria model has been adopted for evaluation of osteolysis and inflammation induced by polyethylene (PE) or metal wear debris. However, this model suffers from several complications. The purpose of our study is to introduce a surgical modification with lower complication rates, thus pro...
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Hindawi Publishing Corporation
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681799/ |
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pubmed-46817992016-01-14 Surgical Modification of the Murine Calvaria Osteolysis Model Al-quhali, Ali Mohammed Sun, Yu Bai, Xizhuang Jin, Zhe Yu, Guibo Research Article The murine calvaria model has been adopted for evaluation of osteolysis and inflammation induced by polyethylene (PE) or metal wear debris. However, this model suffers from several complications. The purpose of our study is to introduce a surgical modification with lower complication rates, thus providing more accurate results. Forty C57/BL6 mice were divided into two groups, both receiving polyethylene particles. Surgical modifications were performed in group 1, and group 2 underwent traditional surgeries. The incidence of fluid leakage was recorded on the operative day. Curst formation, wound dehiscence, and bone exposure were recorded on day 7. Histological osteolysis was demonstrated by HE staining of tissue slices. Micro-CT was used for quantifying evaluation of osteolysis in two groups. Intraoperative fluid leakage was significantly reduced in group 1. Postoperative crust formation, wound dehiscence, and bone exposure were also significantly decreased in group 1. HE staining results revealed obvious osteolysis in group 1 and more obvious osteolysis in group 2. Bone volume fraction (BVF) was (0.32 ± 0.03) in group 1 compared to group 2 (0.24 ± 0.05). Bone mineral density (BMD) was (1.11 ± 0.03) in group 1 compared to group 2 (1.01 ± 0.02). Surgical modifications provide a reliable way for establishment of the murine calvaria osteolysis model. Hindawi Publishing Corporation 2015 2015-12-03 /pmc/articles/PMC4681799/ /pubmed/26769571 http://dx.doi.org/10.1155/2015/802697 Text en Copyright © 2015 Ali Mohammed Al-quhali et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Al-quhali, Ali Mohammed Sun, Yu Bai, Xizhuang Jin, Zhe Yu, Guibo |
| spellingShingle |
Al-quhali, Ali Mohammed Sun, Yu Bai, Xizhuang Jin, Zhe Yu, Guibo Surgical Modification of the Murine Calvaria Osteolysis Model |
| author_facet |
Al-quhali, Ali Mohammed Sun, Yu Bai, Xizhuang Jin, Zhe Yu, Guibo |
| author_sort |
Al-quhali, Ali Mohammed |
| title |
Surgical Modification of the Murine Calvaria Osteolysis Model |
| title_short |
Surgical Modification of the Murine Calvaria Osteolysis Model |
| title_full |
Surgical Modification of the Murine Calvaria Osteolysis Model |
| title_fullStr |
Surgical Modification of the Murine Calvaria Osteolysis Model |
| title_full_unstemmed |
Surgical Modification of the Murine Calvaria Osteolysis Model |
| title_sort |
surgical modification of the murine calvaria osteolysis model |
| description |
The murine calvaria model has been adopted for evaluation of osteolysis and inflammation induced by polyethylene (PE) or metal wear debris. However, this model suffers from several complications. The purpose of our study is to introduce a surgical modification with lower complication rates, thus providing more accurate results. Forty C57/BL6 mice were divided into two groups, both receiving polyethylene particles. Surgical modifications were performed in group 1, and group 2 underwent traditional surgeries. The incidence of fluid leakage was recorded on the operative day. Curst formation, wound dehiscence, and bone exposure were recorded on day 7. Histological osteolysis was demonstrated by HE staining of tissue slices. Micro-CT was used for quantifying evaluation of osteolysis in two groups. Intraoperative fluid leakage was significantly reduced in group 1. Postoperative crust formation, wound dehiscence, and bone exposure were also significantly decreased in group 1. HE staining results revealed obvious osteolysis in group 1 and more obvious osteolysis in group 2. Bone volume fraction (BVF) was (0.32 ± 0.03) in group 1 compared to group 2 (0.24 ± 0.05). Bone mineral density (BMD) was (1.11 ± 0.03) in group 1 compared to group 2 (1.01 ± 0.02). Surgical modifications provide a reliable way for establishment of the murine calvaria osteolysis model. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681799/ |
| _version_ |
1613513535760891904 |