Molecular structures of unbound and transcribing RNA polymerase III
Transcription of genes encoding small structured RNAs such as tRNAs, spliceosomal U6 snRNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. The cryo-EM structures of the S. cerevisiae Pol III elongating c...
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pubmed-46811322016-05-25 Molecular structures of unbound and transcribing RNA polymerase III Hoffmann, Niklas A. Jakobi, Arjen J. Moreno-Morcillo, Maria Glatt, Sebastian Kosinski, Jan Hagen, Wim J. H. Sachse, Carsten Müller, Christoph W. Article Transcription of genes encoding small structured RNAs such as tRNAs, spliceosomal U6 snRNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. The cryo-EM structures of the S. cerevisiae Pol III elongating complex at 3.9 Å resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 Å resolution, respectively, allow for the first time to build a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82/C34/C31 heterotrimer in close proximity to the stalk. The C53/C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets. 2015-11-25 2015-12-10 /pmc/articles/PMC4681132/ /pubmed/26605533 http://dx.doi.org/10.1038/nature16143 Text en Reprints and permissions information is available at www.nature.com/reprints (http://www.nature.com/reprints) . Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
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Open Access Journal |
institution_category |
Foreign Institution |
institution |
US National Center for Biotechnology Information |
building |
NCBI PubMed |
collection |
Online Access |
language |
English |
format |
Online |
author |
Hoffmann, Niklas A. Jakobi, Arjen J. Moreno-Morcillo, Maria Glatt, Sebastian Kosinski, Jan Hagen, Wim J. H. Sachse, Carsten Müller, Christoph W. |
spellingShingle |
Hoffmann, Niklas A. Jakobi, Arjen J. Moreno-Morcillo, Maria Glatt, Sebastian Kosinski, Jan Hagen, Wim J. H. Sachse, Carsten Müller, Christoph W. Molecular structures of unbound and transcribing RNA polymerase III |
author_facet |
Hoffmann, Niklas A. Jakobi, Arjen J. Moreno-Morcillo, Maria Glatt, Sebastian Kosinski, Jan Hagen, Wim J. H. Sachse, Carsten Müller, Christoph W. |
author_sort |
Hoffmann, Niklas A. |
title |
Molecular structures of unbound and transcribing RNA polymerase III |
title_short |
Molecular structures of unbound and transcribing RNA polymerase III |
title_full |
Molecular structures of unbound and transcribing RNA polymerase III |
title_fullStr |
Molecular structures of unbound and transcribing RNA polymerase III |
title_full_unstemmed |
Molecular structures of unbound and transcribing RNA polymerase III |
title_sort |
molecular structures of unbound and transcribing rna polymerase iii |
description |
Transcription of genes encoding small structured RNAs such as tRNAs, spliceosomal U6 snRNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. The cryo-EM structures of the S. cerevisiae Pol III elongating complex at 3.9 Å resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 Å resolution, respectively, allow for the first time to build a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82/C34/C31 heterotrimer in close proximity to the stalk. The C53/C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets. |
publishDate |
2015 |
url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4681132/ |
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1613513365826568192 |