Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus

Streptococcus gallolyticus subspecies gallolyticus (S. gallolyticus) can colonise the gastrointestinal tract of humans and animals and is known to cause similar infections in both humans and animals. Data about the spread or prevalence in farm animals are missing. In this study, Trypton Soya Agar w...

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Main Authors:	Schulz, Jochen, Dumke, Jessika, Hinse, Dennis, Dreier, Jens, Habig, Christin, Kemper, Nicole
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675529/

id	pubmed-4675529
recordtype	oai_dc
spelling	pubmed-46755292015-12-31 Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus Schulz, Jochen Dumke, Jessika Hinse, Dennis Dreier, Jens Habig, Christin Kemper, Nicole Research Article Streptococcus gallolyticus subspecies gallolyticus (S. gallolyticus) can colonise the gastrointestinal tract of humans and animals and is known to cause similar infections in both humans and animals. Data about the spread or prevalence in farm animals are missing. In this study, Trypton Soya Agar was modified to a selective medium enabling the isolation and quantification of S. gallolyticus from faecal samples. The bacterium was observed in 82 out of 91 faecal samples obtained from 18 different organic turkey flocks. The prevalence of shedding birds was estimated by the number of positive fresh droppings and reached up to 100% on most farms. Furthermore, for the first time S. gallolyticus was quantified in faeces from poultry flocks. The median of colony forming units (CFU) per gramme faeces was 3.6 x 105CFU/g. Typing of one isolate from each positive faecal sample by multilocus sequence typing delivered 24 sequence types (STs). Most of the isolates belonged to the clonal complex CC58. The same STs of this complex were detected in up to six different flocks. Partly, these flocks were located in various regions and stocked with varying breeding lines. Regarding the biochemical profiles of the same STs from different farms, the results did not contradict a spread of specific STs in the organic turkey production. Moreover, checking the pubMLST database revealed that STs found in this study were also found in other animal species and in humans. The high detection rate and the number of S. gallolyticus in turkey faeces indicate that this bacterium probably belongs to the common microbiota of the gastrointestinal tract of turkeys from organic flocks. Furthermore, the findings of this study support the suggestion of a possible interspecies transmission. Public Library of Science 2015-12-10 /pmc/articles/PMC4675529/ /pubmed/26657757 http://dx.doi.org/10.1371/journal.pone.0144412 Text en © 2015 Schulz et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Schulz, Jochen Dumke, Jessika Hinse, Dennis Dreier, Jens Habig, Christin Kemper, Nicole
spellingShingle	Schulz, Jochen Dumke, Jessika Hinse, Dennis Dreier, Jens Habig, Christin Kemper, Nicole Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus
author_facet	Schulz, Jochen Dumke, Jessika Hinse, Dennis Dreier, Jens Habig, Christin Kemper, Nicole
author_sort	Schulz, Jochen
title	Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus
title_short	Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus
title_full	Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus
title_fullStr	Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus
title_full_unstemmed	Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus
title_sort	organic turkey flocks: a reservoir of streptococcus gallolyticus subspecies gallolyticus
description	Streptococcus gallolyticus subspecies gallolyticus (S. gallolyticus) can colonise the gastrointestinal tract of humans and animals and is known to cause similar infections in both humans and animals. Data about the spread or prevalence in farm animals are missing. In this study, Trypton Soya Agar was modified to a selective medium enabling the isolation and quantification of S. gallolyticus from faecal samples. The bacterium was observed in 82 out of 91 faecal samples obtained from 18 different organic turkey flocks. The prevalence of shedding birds was estimated by the number of positive fresh droppings and reached up to 100% on most farms. Furthermore, for the first time S. gallolyticus was quantified in faeces from poultry flocks. The median of colony forming units (CFU) per gramme faeces was 3.6 x 105CFU/g. Typing of one isolate from each positive faecal sample by multilocus sequence typing delivered 24 sequence types (STs). Most of the isolates belonged to the clonal complex CC58. The same STs of this complex were detected in up to six different flocks. Partly, these flocks were located in various regions and stocked with varying breeding lines. Regarding the biochemical profiles of the same STs from different farms, the results did not contradict a spread of specific STs in the organic turkey production. Moreover, checking the pubMLST database revealed that STs found in this study were also found in other animal species and in humans. The high detection rate and the number of S. gallolyticus in turkey faeces indicate that this bacterium probably belongs to the common microbiota of the gastrointestinal tract of turkeys from organic flocks. Furthermore, the findings of this study support the suggestion of a possible interspecies transmission.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4675529/
_version_	1613511708060418048

Organic Turkey Flocks: A Reservoir of Streptococcus gallolyticus subspecies gallolyticus

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