Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies

Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies

Since 2010, studies on the diagnostic accuracy of COBAS TaqMan MTB (CTM) have been frequently reported with an unignorable discrepancy. The key inclusion criterion for this systematic review was original studies that could provide sufficient data for calculating the sensitivity and the specificity o...

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Main Authors: Horita, Nobuyuki, Yamamoto, Masaki, Sato, Takashi, Tsukahara, Toshinori, Nagakura, Hideyuki, Tashiro, Ken, Shibata, Yuji, Watanabe, Hiroki, Nagai, Kenjiro, Nakashima, Kentaro, Ushio, Ryota, Ikeda, Misako, Sakamaki, Kentaro, Yoshiyama, Takashi, Kaneko, Takeshi
Format: Online
Language:English
Published: Nature Publishing Group 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673449/
id pubmed-4673449
recordtype oai_dc
spelling pubmed-46734492015-12-14 Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies Horita, Nobuyuki Yamamoto, Masaki Sato, Takashi Tsukahara, Toshinori Nagakura, Hideyuki Tashiro, Ken Shibata, Yuji Watanabe, Hiroki Nagai, Kenjiro Nakashima, Kentaro Ushio, Ryota Ikeda, Misako Sakamaki, Kentaro Yoshiyama, Takashi Kaneko, Takeshi Article Since 2010, studies on the diagnostic accuracy of COBAS TaqMan MTB (CTM) have been frequently reported with an unignorable discrepancy. The key inclusion criterion for this systematic review was original studies that could provide sufficient data for calculating the sensitivity and the specificity of CTM for M tuberculosis (TB) or M tuberculosis complex. The reference test was Mycobacterium culture. We used bivariate model for meta-analyses. Of the 201 candidate articles, we finally identified 17 eligible articles.Concerning the respiratory specimens, 1900 culture positive specimens and 20983 culture negative specimens from 15 studies were assessed. This provided the summary estimate sensitivity of 0.808 (95% CI 0.758–0.850) and the summary estimate specificity of 0.990 (95% CI 0.981–0.994). The area under curve was 0.956. The diagnostic odds ratio was 459 (95% CI 261–805, I2 26%). For the smear positive respiratory specimens, the sensitivity was 0.952 (95% CI 0.926–0.969) and the specificity was 0.916 (95% CI 0.797–0.968). For the smear negative respiratory specimens, the sensitivity and the specificity were 0.600 (95% CI 0.459–0.726) and 0.989 (95% CI 0.981–0.993), respectively. The diagnostic accuracy was poorer for the non-respiratory specimens, than for the respiratory specimens, but was acceptable. We believe that the information obtained from this study will aid physicians’ decision making. Nature Publishing Group 2015-12-09 /pmc/articles/PMC4673449/ /pubmed/26648113 http://dx.doi.org/10.1038/srep18113 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Horita, Nobuyuki
Yamamoto, Masaki
Sato, Takashi
Tsukahara, Toshinori
Nagakura, Hideyuki
Tashiro, Ken
Shibata, Yuji
Watanabe, Hiroki
Nagai, Kenjiro
Nakashima, Kentaro
Ushio, Ryota
Ikeda, Misako
Sakamaki, Kentaro
Yoshiyama, Takashi
Kaneko, Takeshi
spellingShingle Horita, Nobuyuki
Yamamoto, Masaki
Sato, Takashi
Tsukahara, Toshinori
Nagakura, Hideyuki
Tashiro, Ken
Shibata, Yuji
Watanabe, Hiroki
Nagai, Kenjiro
Nakashima, Kentaro
Ushio, Ryota
Ikeda, Misako
Sakamaki, Kentaro
Yoshiyama, Takashi
Kaneko, Takeshi
Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies
author_facet Horita, Nobuyuki
Yamamoto, Masaki
Sato, Takashi
Tsukahara, Toshinori
Nagakura, Hideyuki
Tashiro, Ken
Shibata, Yuji
Watanabe, Hiroki
Nagai, Kenjiro
Nakashima, Kentaro
Ushio, Ryota
Ikeda, Misako
Sakamaki, Kentaro
Yoshiyama, Takashi
Kaneko, Takeshi
author_sort Horita, Nobuyuki
title Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies
title_short Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies
title_full Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies
title_fullStr Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies
title_full_unstemmed Sensitivity and specificity of Cobas TaqMan MTB real-time polymerase chain reaction for culture-proven Mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 Studies
title_sort sensitivity and specificity of cobas taqman mtb real-time polymerase chain reaction for culture-proven mycobacterium tuberculosis: meta-analysis of 26999 specimens from 17 studies
description Since 2010, studies on the diagnostic accuracy of COBAS TaqMan MTB (CTM) have been frequently reported with an unignorable discrepancy. The key inclusion criterion for this systematic review was original studies that could provide sufficient data for calculating the sensitivity and the specificity of CTM for M tuberculosis (TB) or M tuberculosis complex. The reference test was Mycobacterium culture. We used bivariate model for meta-analyses. Of the 201 candidate articles, we finally identified 17 eligible articles.Concerning the respiratory specimens, 1900 culture positive specimens and 20983 culture negative specimens from 15 studies were assessed. This provided the summary estimate sensitivity of 0.808 (95% CI 0.758–0.850) and the summary estimate specificity of 0.990 (95% CI 0.981–0.994). The area under curve was 0.956. The diagnostic odds ratio was 459 (95% CI 261–805, I2 26%). For the smear positive respiratory specimens, the sensitivity was 0.952 (95% CI 0.926–0.969) and the specificity was 0.916 (95% CI 0.797–0.968). For the smear negative respiratory specimens, the sensitivity and the specificity were 0.600 (95% CI 0.459–0.726) and 0.989 (95% CI 0.981–0.993), respectively. The diagnostic accuracy was poorer for the non-respiratory specimens, than for the respiratory specimens, but was acceptable. We believe that the information obtained from this study will aid physicians’ decision making.
publisher Nature Publishing Group
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4673449/
_version_ 1613510999551246336

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