Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats
Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since el...
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Associação Brasileira de Divulgação Científica
2015
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671533/ |
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pubmed-46715332015-12-22 Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats Zotz, T.G.G. de Paula, J.B. Clinical Investigation Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction was performed by bone marrow implantation in both quadriceps of the animals, which were then divided into 3 groups: control (CG), transcutaneous electrical nerve stimulation (TENS) group (TG), and functional electrical stimulation (FES) group (FG) with 12 rats each. All animals were anesthetized and electrically stimulated twice per week, for 35 days from induction day. After this period, another blood sample was collected and quadriceps muscles were bilaterally removed for histological and calcium analysis and the rats were killed. Calcium levels in muscles showed significantly lower results when comparing TG and FG (P<0.001) and between TG and CG (P<0.001). Qualitative histological analyses confirmed 100% HO in FG and CG, while in TG the HO was detected in 54.5% of the animals. The effects of the muscle contractions caused by FES increased HO, while anti-inflammatory effects of TENS reduced HO. Associação Brasileira de Divulgação Científica 2015-08-18 /pmc/articles/PMC4671533/ /pubmed/26292223 http://dx.doi.org/10.1590/1414-431X20153426 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Zotz, T.G.G. de Paula, J.B. |
| spellingShingle |
Zotz, T.G.G. de Paula, J.B. Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats |
| author_facet |
Zotz, T.G.G. de Paula, J.B. |
| author_sort |
Zotz, T.G.G. |
| title |
Influence of transcutaneous electrical stimulation on heterotopic
ossification: an experimental study in Wistar rats |
| title_short |
Influence of transcutaneous electrical stimulation on heterotopic
ossification: an experimental study in Wistar rats |
| title_full |
Influence of transcutaneous electrical stimulation on heterotopic
ossification: an experimental study in Wistar rats |
| title_fullStr |
Influence of transcutaneous electrical stimulation on heterotopic
ossification: an experimental study in Wistar rats |
| title_full_unstemmed |
Influence of transcutaneous electrical stimulation on heterotopic
ossification: an experimental study in Wistar rats |
| title_sort |
influence of transcutaneous electrical stimulation on heterotopic
ossification: an experimental study in wistar rats |
| description |
Heterotopic ossification (HO) is a metaplastic biological process in which there is
newly formed bone in soft tissues, resulting in joint mobility deficit and pain.
Different treatment modalities have been tried to prevent HO development, but there
is no consensus on a therapeutic approach. Since electrical stimulation is a widely
used resource in physiotherapy practice to stimulate joint mobility, with analgesic
and anti-inflammatory effects, its usefulness for HO treatment was investigated. We
aimed to identify the influence of electrical stimulation on induced HO in Wistar
rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized
for blood sampling before HO induction, to quantify the serum alkaline phosphatase.
HO induction was performed by bone marrow implantation in both quadriceps of the
animals, which were then divided into 3 groups: control (CG), transcutaneous
electrical nerve stimulation (TENS) group (TG), and functional electrical stimulation
(FES) group (FG) with 12 rats each. All animals were anesthetized and electrically
stimulated twice per week, for 35 days from induction day. After this period, another
blood sample was collected and quadriceps muscles were bilaterally removed for
histological and calcium analysis and the rats were killed. Calcium levels in muscles
showed significantly lower results when comparing TG and FG (P<0.001) and between
TG and CG (P<0.001). Qualitative histological analyses confirmed 100% HO in FG and
CG, while in TG the HO was detected in 54.5% of the animals. The effects of the
muscle contractions caused by FES increased HO, while anti-inflammatory effects of
TENS reduced HO. |
| publisher |
Associação Brasileira de Divulgação Científica |
| publishDate |
2015 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671533/ |
| _version_ |
1613510186905894912 |