Quantifying NAD(P)H production in the upper Entner–Doudoroff pathway from Pseudomonas putida KT2440
•The first kinetic characterization of PputG6PDH-1 is presented.•The relative production of NADH and NADPH by PputG6PDH-1 is quantified.•The stoichiometric matrix of in silico metabolic models for Pseudomonas putida must be modified.
Main Authors: | , , , , , , |
---|---|
Format: | Online |
Language: | English |
Published: |
Elsevier
2015
|
Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4669411/ |