Molecular Diagnosis of Pathogenic Sporothrix Species

Sporotrichosis, a disease caused by infections from Sporothrix species, primarily affects warm-blooded animals, particularly humans and cats. Sporotrichosis is emerging as a global threat, with high incidences in Brazil and China. The gold standard for diagnosing sporotrichosis is microscopic charac...

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Main Authors:	Rodrigues, Anderson Messias, de Hoog, G. Sybren, de Camargo, Zoilo Pires
Format:	Online
Language:	English
Published:	Public Library of Science 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666615/

id	pubmed-4666615
recordtype	oai_dc
spelling	pubmed-46666152015-12-10 Molecular Diagnosis of Pathogenic Sporothrix Species Rodrigues, Anderson Messias de Hoog, G. Sybren de Camargo, Zoilo Pires Research Article Sporotrichosis, a disease caused by infections from Sporothrix species, primarily affects warm-blooded animals, particularly humans and cats. Sporotrichosis is emerging as a global threat, with high incidences in Brazil and China. The gold standard for diagnosing sporotrichosis is microscopic characterization of the pathogen isolated in culture. This methodology is tedious and time-consuming. Moreover, closely related Sporothrix species are often misidentified, due to similar phenotypic characteristics. The introduction of dissimilar species with specific geographic distributions, host predilections, virulence, and antifungal susceptibilities, has made species-level identification of Sporothrix mandatory. To facilitate meeting this requirement, we developed a PCR-based method for detecting and identifying Sporothrix species. We designed species-specific primers for identifying S. brasiliensis, S. schenckii, S. globosa, S. mexicana, S. pallida, and Ophiostoma stenoceras. With this method, we could detect as little as 1 pg and 10 fg (depending on the species) of Sporothrix DNA derived from isolated cultures. Furthermore, we successfully detected S. brasiliensis and S. schenckii DNA in tissue samples derived from a murine model of disseminated sporotrichosis. These species-specific primers can be applied in epidemiology, clinical diagnosis, and experimental studies of sporotrichosis. Improvements in early diagnosis and surveillance systems may facilitate rapid identification and control of future outbreaks. Public Library of Science 2015-12-01 /pmc/articles/PMC4666615/ /pubmed/26623643 http://dx.doi.org/10.1371/journal.pntd.0004190 Text en © 2015 Rodrigues et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Rodrigues, Anderson Messias de Hoog, G. Sybren de Camargo, Zoilo Pires
spellingShingle	Rodrigues, Anderson Messias de Hoog, G. Sybren de Camargo, Zoilo Pires Molecular Diagnosis of Pathogenic Sporothrix Species
author_facet	Rodrigues, Anderson Messias de Hoog, G. Sybren de Camargo, Zoilo Pires
author_sort	Rodrigues, Anderson Messias
title	Molecular Diagnosis of Pathogenic Sporothrix Species
title_short	Molecular Diagnosis of Pathogenic Sporothrix Species
title_full	Molecular Diagnosis of Pathogenic Sporothrix Species
title_fullStr	Molecular Diagnosis of Pathogenic Sporothrix Species
title_full_unstemmed	Molecular Diagnosis of Pathogenic Sporothrix Species
title_sort	molecular diagnosis of pathogenic sporothrix species
description	Sporotrichosis, a disease caused by infections from Sporothrix species, primarily affects warm-blooded animals, particularly humans and cats. Sporotrichosis is emerging as a global threat, with high incidences in Brazil and China. The gold standard for diagnosing sporotrichosis is microscopic characterization of the pathogen isolated in culture. This methodology is tedious and time-consuming. Moreover, closely related Sporothrix species are often misidentified, due to similar phenotypic characteristics. The introduction of dissimilar species with specific geographic distributions, host predilections, virulence, and antifungal susceptibilities, has made species-level identification of Sporothrix mandatory. To facilitate meeting this requirement, we developed a PCR-based method for detecting and identifying Sporothrix species. We designed species-specific primers for identifying S. brasiliensis, S. schenckii, S. globosa, S. mexicana, S. pallida, and Ophiostoma stenoceras. With this method, we could detect as little as 1 pg and 10 fg (depending on the species) of Sporothrix DNA derived from isolated cultures. Furthermore, we successfully detected S. brasiliensis and S. schenckii DNA in tissue samples derived from a murine model of disseminated sporotrichosis. These species-specific primers can be applied in epidemiology, clinical diagnosis, and experimental studies of sporotrichosis. Improvements in early diagnosis and surveillance systems may facilitate rapid identification and control of future outbreaks.
publisher	Public Library of Science
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666615/
_version_	1613508534147743744

Molecular Diagnosis of Pathogenic Sporothrix Species

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