Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests

Haemonchus contortus is a blood-sucking parasite causing the presence of faecal occult blood (FOB). The objective was to study three different FOB tests in order to have a new indicator of H. contortus infection in sheep that could be included in the genetic evaluation system as an alternative selec...

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Main Authors:	Rodríguez, A.V., Goldberg, V., Viotti, H., Ciappesoni, G.
Format:	Online
Language:	English
Published:	Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663806/

id	pubmed-4663806
recordtype	oai_dc
spelling	pubmed-46638062015-11-30 Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests Rodríguez, A.V. Goldberg, V. Viotti, H. Ciappesoni, G. Original Article Haemonchus contortus is a blood-sucking parasite causing the presence of faecal occult blood (FOB). The objective was to study three different FOB tests in order to have a new indicator of H. contortus infection in sheep that could be included in the genetic evaluation system as an alternative selection criterion to faecal worm egg count (FEC). A total of 29 Corriedale lambs were experimentally infected with 10.000 larvae of H. contortus. Stool samples were recorded for FEC and FOB tests (Hexagon, Hematest® and Multistix®), blood for packed cell volume (PCV), haemoglobin, white and red blood cell count (RBC), and FAMACHA© for scoring anaemia. At the end of the experiment lambs were slaughtered to worm burden count. Field infection was achieved in 309 Merino lambs under natural parasite challenge. FEC data were normalized through logarithmic transformation (LnFEC). Pearson correlation was estimated to examine the relationship between all traits. The three tests were able to detect the presence of FOB at day 11. FEC, PCV and RBC decreased to sub-normal values from day 18. FAMACHA© score 3 was considered to be indicative of anaemia. Most of the correlations were of high magnitude, with the exception of Multistix® test that was moderately correlated with haematological parameters, LnFEC and FEC. In field infection, most samples were negative to FOB tests and the correlations were lower than those calculated under experimental infection. In conclusion, FOB tests were able to detect haemonchosis earlier than FEC under high experimental parasite challenge. However, they were not able to detect FOB under natural mixed parasite challenge. FAMACHA© and PCV demonstrated to be good indicators of Haemonchosis, having moderate to high correlations with FEC. Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology 2015 2015-07-11 /pmc/articles/PMC4663806/ /pubmed/26623372 Text en Copyright: © Open Veterinary Journal http://creativecommons.org/licenses/by-nc-sa/4.0 Open Veterinary Journal is licensed under a Creative Commons Attribution 4.0 International License.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Rodríguez, A.V. Goldberg, V. Viotti, H. Ciappesoni, G.
spellingShingle	Rodríguez, A.V. Goldberg, V. Viotti, H. Ciappesoni, G. Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
author_facet	Rodríguez, A.V. Goldberg, V. Viotti, H. Ciappesoni, G.
author_sort	Rodríguez, A.V.
title	Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
title_short	Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
title_full	Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
title_fullStr	Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
title_full_unstemmed	Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests
title_sort	early detection of haemonchus contortus infection in sheep using three different faecal occult blood tests
description	Haemonchus contortus is a blood-sucking parasite causing the presence of faecal occult blood (FOB). The objective was to study three different FOB tests in order to have a new indicator of H. contortus infection in sheep that could be included in the genetic evaluation system as an alternative selection criterion to faecal worm egg count (FEC). A total of 29 Corriedale lambs were experimentally infected with 10.000 larvae of H. contortus. Stool samples were recorded for FEC and FOB tests (Hexagon, Hematest® and Multistix®), blood for packed cell volume (PCV), haemoglobin, white and red blood cell count (RBC), and FAMACHA© for scoring anaemia. At the end of the experiment lambs were slaughtered to worm burden count. Field infection was achieved in 309 Merino lambs under natural parasite challenge. FEC data were normalized through logarithmic transformation (LnFEC). Pearson correlation was estimated to examine the relationship between all traits. The three tests were able to detect the presence of FOB at day 11. FEC, PCV and RBC decreased to sub-normal values from day 18. FAMACHA© score 3 was considered to be indicative of anaemia. Most of the correlations were of high magnitude, with the exception of Multistix® test that was moderately correlated with haematological parameters, LnFEC and FEC. In field infection, most samples were negative to FOB tests and the correlations were lower than those calculated under experimental infection. In conclusion, FOB tests were able to detect haemonchosis earlier than FEC under high experimental parasite challenge. However, they were not able to detect FOB under natural mixed parasite challenge. FAMACHA© and PCV demonstrated to be good indicators of Haemonchosis, having moderate to high correlations with FEC.
publisher	Faculty of Veterinary Medicine, University of Tripoli and Libyan Authority for Research, Science and Technology
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663806/
_version_	1613507655875166208

Early detection of Haemonchus contortus infection in sheep using three different faecal occult blood tests

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