Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes

Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes

Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolase...

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Main Authors: Viens, Pascal, Lacombe-Harvey, Marie-Ève, Brzezinski, Ryszard
Format: Online
Language:English
Published: MDPI 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663542/
id pubmed-4663542
recordtype oai_dc
spelling pubmed-46635422015-12-10 Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes Viens, Pascal Lacombe-Harvey, Marie-Ève Brzezinski, Ryszard Review Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging to family GH46 are among the best-studied chitosanases, with four crystallography-derived structures available and more than forty enzymes studied at the biochemical level. They were also subjected to numerous site-directed mutagenesis studies, unraveling the molecular mechanisms of hydrolysis. This review is focused on the taxonomic distribution of GH46 proteins, their multi-modular character, the structure-function relationships and their biological functions in the host organisms. MDPI 2015-10-28 /pmc/articles/PMC4663542/ /pubmed/26516868 http://dx.doi.org/10.3390/md13116566 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Viens, Pascal
Lacombe-Harvey, Marie-Ève
Brzezinski, Ryszard
spellingShingle Viens, Pascal
Lacombe-Harvey, Marie-Ève
Brzezinski, Ryszard
Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
author_facet Viens, Pascal
Lacombe-Harvey, Marie-Ève
Brzezinski, Ryszard
author_sort Viens, Pascal
title Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
title_short Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
title_full Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
title_fullStr Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
title_full_unstemmed Chitosanases from Family 46 of Glycoside Hydrolases: From Proteins to Phenotypes
title_sort chitosanases from family 46 of glycoside hydrolases: from proteins to phenotypes
description Chitosanases, enzymes that catalyze the endo-hydrolysis of glycolytic links in chitosan, are the subject of numerous studies as biotechnological tools to generate low molecular weight chitosan (LMWC) or chitosan oligosaccharides (CHOS) from native, high molecular weight chitosan. Glycoside hydrolases belonging to family GH46 are among the best-studied chitosanases, with four crystallography-derived structures available and more than forty enzymes studied at the biochemical level. They were also subjected to numerous site-directed mutagenesis studies, unraveling the molecular mechanisms of hydrolysis. This review is focused on the taxonomic distribution of GH46 proteins, their multi-modular character, the structure-function relationships and their biological functions in the host organisms.
publisher MDPI
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663542/
_version_ 1613507546838990848

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