A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells

The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) ce...

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Main Authors:	Ogaki, Soichiro, Morooka, Mayu, Otera, Kaito, Kume, Shoen
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663490/

id	pubmed-4663490
recordtype	oai_dc
spelling	pubmed-46634902015-12-03 A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells Ogaki, Soichiro Morooka, Mayu Otera, Kaito Kume, Shoen Article The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells. In the presence of dimethyl sulfoxide (DMSO), a low concentration of Activin at 6.25 ng/ml is sufficient to give a similar differentiation efficiency with that using Activin at 100 ng/ml at the presence of Wnt activator. In the presence of DMSO, Activin at low concentration triggered hiPS cells to undergo differentiation through G1 arrest, reduce apoptosis, and potentiate activation of downstream targets, such as SMAD2 phosphorylation and SOX17 expression. This increased differentiation into CDX2 + SOX17 + DE cells. The present differentiation procedure therefore permits rapid and efficient derivation of DE cells, capable of differentiating into intestinal epithelium upon BIO and DAPT treatment and of giving rise to functional cells, such as enterocytes. Nature Publishing Group 2015-11-30 /pmc/articles/PMC4663490/ /pubmed/26616277 http://dx.doi.org/10.1038/srep17297 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ogaki, Soichiro Morooka, Mayu Otera, Kaito Kume, Shoen
spellingShingle	Ogaki, Soichiro Morooka, Mayu Otera, Kaito Kume, Shoen A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
author_facet	Ogaki, Soichiro Morooka, Mayu Otera, Kaito Kume, Shoen
author_sort	Ogaki, Soichiro
title	A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
title_short	A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
title_full	A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
title_fullStr	A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
title_full_unstemmed	A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
title_sort	cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells
description	The human intestinal epithelium is a useful model for pharmacological studies of absorption, metabolism, drug interactions, and toxicology, as well as for studies of developmental biology. We established a rapid and cost effective system for differentiation of human induced pluripotent stem (iPS) cells into definitive endoderm (DE) cells. In the presence of dimethyl sulfoxide (DMSO), a low concentration of Activin at 6.25 ng/ml is sufficient to give a similar differentiation efficiency with that using Activin at 100 ng/ml at the presence of Wnt activator. In the presence of DMSO, Activin at low concentration triggered hiPS cells to undergo differentiation through G1 arrest, reduce apoptosis, and potentiate activation of downstream targets, such as SMAD2 phosphorylation and SOX17 expression. This increased differentiation into CDX2 + SOX17 + DE cells. The present differentiation procedure therefore permits rapid and efficient derivation of DE cells, capable of differentiating into intestinal epithelium upon BIO and DAPT treatment and of giving rise to functional cells, such as enterocytes.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4663490/
_version_	1613507518657462272

A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells

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