West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey

West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey

Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling...

Full description

Bibliographic Details
Main Authors: Hadjichristodoulou, Christos, Pournaras, Spyros, Mavrouli, Maria, Marka, Andriani, Tserkezou, Persefoni, Baka, Agoritsa, Billinis, Charalambos, Katsioulis, Antonios, Psaroulaki, Anna, Papa, Anna, Papadopoulos, Nikos, Mamuris, Zissis, Tsakris, Athanasios, Kremastinou, Jenny
Format: Online
Language:English
Published: Public Library of Science 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659653/
id pubmed-4659653
recordtype oai_dc
spelling pubmed-46596532015-12-02 West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey Hadjichristodoulou, Christos Pournaras, Spyros Mavrouli, Maria Marka, Andriani Tserkezou, Persefoni Baka, Agoritsa Billinis, Charalambos Katsioulis, Antonios Psaroulaki, Anna Papa, Anna Papadopoulos, Nikos Mamuris, Zissis Tsakris, Athanasios Kremastinou, Jenny Research Article Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme–Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7–2.6) and 1.5% (95% C.I.: 1.2–2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421–1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease. Public Library of Science 2015-11-25 /pmc/articles/PMC4659653/ /pubmed/26605539 http://dx.doi.org/10.1371/journal.pone.0143803 Text en © 2015 Hadjichristodoulou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Hadjichristodoulou, Christos
Pournaras, Spyros
Mavrouli, Maria
Marka, Andriani
Tserkezou, Persefoni
Baka, Agoritsa
Billinis, Charalambos
Katsioulis, Antonios
Psaroulaki, Anna
Papa, Anna
Papadopoulos, Nikos
Mamuris, Zissis
Tsakris, Athanasios
Kremastinou, Jenny
spellingShingle Hadjichristodoulou, Christos
Pournaras, Spyros
Mavrouli, Maria
Marka, Andriani
Tserkezou, Persefoni
Baka, Agoritsa
Billinis, Charalambos
Katsioulis, Antonios
Psaroulaki, Anna
Papa, Anna
Papadopoulos, Nikos
Mamuris, Zissis
Tsakris, Athanasios
Kremastinou, Jenny
West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
author_facet Hadjichristodoulou, Christos
Pournaras, Spyros
Mavrouli, Maria
Marka, Andriani
Tserkezou, Persefoni
Baka, Agoritsa
Billinis, Charalambos
Katsioulis, Antonios
Psaroulaki, Anna
Papa, Anna
Papadopoulos, Nikos
Mamuris, Zissis
Tsakris, Athanasios
Kremastinou, Jenny
author_sort Hadjichristodoulou, Christos
title West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
title_short West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
title_full West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
title_fullStr West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
title_full_unstemmed West Nile Virus Seroprevalence in the Greek Population in 2013: A Nationwide Cross-Sectional Survey
title_sort west nile virus seroprevalence in the greek population in 2013: a nationwide cross-sectional survey
description Cases of West Nile Virus (WNV) disease were recorded for three consecutive years in Greece following the year 2010 outbreak. A cross-sectional serologic survey was conducted to estimate the WNV seroprevalence and assess the ratio of infection to neuroinvasive disease. A stratified left-over sampling methodology was used including age and residence strata. A total of 3,962 serum samples was collected and tested for WNV Immunoglobulin G (IgG) antibodies by Enzyme–Linked Immunosorbent Assay (ELISA). All positive samples were further tested by Plaque Reduction Neutralization Test (PRNT) and WNV Immunoglobulin M (IgM) antibodies. WNV IgG antibodies were detected in 82 samples and 61 were also positive in PRNT representing a weighted seroprevalence of 2.1% (95% C.I.: 1.7–2.6) and 1.5% (95% C.I.: 1.2–2.0), respectively. Multivariable analysis showed that seroprevalence was associated with age and residence. The overall ratio of neuroinvasive disease to infected persons was estimated at 1:376 (95% C.I.: 1:421–1:338), while the elderly people had the highest ratio. This nationwide study provided valuable data regarding the epidemiology of WNV in Greece based on the fact that elderly people have higher risk of being both infected and having severe disease.
publisher Public Library of Science
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4659653/
_version_ 1613505929667411968

Similar Items