Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus

Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus

An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most signif...

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Main Authors: Liguori, Rossana, Ionata, Elena, Marcolongo, Loredana, Vandenberghe, Luciana Porto de Souza, La Cara, Francesco, Faraco, Vincenza
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2015
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652331/
id pubmed-4652331
recordtype oai_dc
spelling pubmed-46523312015-12-02 Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus Liguori, Rossana Ionata, Elena Marcolongo, Loredana Vandenberghe, Luciana Porto de Souza La Cara, Francesco Faraco, Vincenza Research Article An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated A. donax. As the most significant influence during the enzymatic hydrolysis of A. donax was exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion by P. ostreatus cellulase and xylanase was analyzed by a 33 factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes. Hindawi Publishing Corporation 2015 2015-11-05 /pmc/articles/PMC4652331/ /pubmed/26634214 http://dx.doi.org/10.1155/2015/951871 Text en Copyright © 2015 Rossana Liguori et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Liguori, Rossana
Ionata, Elena
Marcolongo, Loredana
Vandenberghe, Luciana Porto de Souza
La Cara, Francesco
Faraco, Vincenza
spellingShingle Liguori, Rossana
Ionata, Elena
Marcolongo, Loredana
Vandenberghe, Luciana Porto de Souza
La Cara, Francesco
Faraco, Vincenza
Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
author_facet Liguori, Rossana
Ionata, Elena
Marcolongo, Loredana
Vandenberghe, Luciana Porto de Souza
La Cara, Francesco
Faraco, Vincenza
author_sort Liguori, Rossana
title Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
title_short Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
title_full Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
title_fullStr Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
title_full_unstemmed Optimization of Arundo donax Saccharification by (Hemi)cellulolytic Enzymes from Pleurotus ostreatus
title_sort optimization of arundo donax saccharification by (hemi)cellulolytic enzymes from pleurotus ostreatus
description An enzymatic mixture of cellulases and xylanases was produced by Pleurotus ostreatus using microcrystalline cellulose as inducer, partially characterized and tested in the statistical analysis of Arundo donax bioconversion. The Plackett-Burman screening design was applied to identify the most significant parameters for the enzymatic hydrolysis of pretreated A. donax. As the most significant influence during the enzymatic hydrolysis of A. donax was exercised by the temperature (°C), pH, and time, the combined effect of these factors in the bioconversion by P. ostreatus cellulase and xylanase was analyzed by a 33 factorial experimental design. It is worth noting that the best result of 480.10 mg of sugars/gds, obtained at 45°C, pH 3.5, and 96 hours of incubation, was significant also when compared with the results previously reached by process optimization with commercial enzymes.
publisher Hindawi Publishing Corporation
publishDate 2015
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4652331/
_version_ 1613503658817748992

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