miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2

Colorectal cancer (CRC) is one of the most common cancers worldwide, with a particularly high incidence in developed countries. Distant metastasis and recurrence are the main causes of CRC-related deaths. MicroRNAs (miRNAs) in the serum make them potential biomarkers for cancers, as reported in seru...

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Main Authors:	Sheng, L, He, P, Yang, X, Zhou, M, Feng, Q
Format:	Online
Language:	English
Published:	Nature Publishing Group 2015
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650731/

id	pubmed-4650731
recordtype	oai_dc
spelling	pubmed-46507312015-12-02 miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2 Sheng, L He, P Yang, X Zhou, M Feng, Q Original Article Colorectal cancer (CRC) is one of the most common cancers worldwide, with a particularly high incidence in developed countries. Distant metastasis and recurrence are the main causes of CRC-related deaths. MicroRNAs (miRNAs) in the serum make them potential biomarkers for cancers, as reported in serum or tumor tissues from CRC patients. In this study, we found that miR-612 expression was significantly lower in CRC tissues or cells compared with peritumor tissues or normal cells, and lower in metastatic CRC specimens compared with non-metastatic specimens, whereas AKT2 exhibited opposite trend. Gain-of-function and loss-of-function assays showed that miR-612 inhibited CRC cell proliferation and migration in vitro by Cell Counting Kit-8 and transwell assays. Further analysis revealed that miR-612 directly suppressed AKT2, which in turn inhibited the downstream epithelial–mesenchymal transition-related signaling pathway. These results were additionally validated in vivo by tumorigenesis and liver metastasis experiments. The results of this study suggested a critical role of miR-612 in the development of CRC. Nature Publishing Group 2015-07 2015-07-09 /pmc/articles/PMC4650731/ /pubmed/26158514 http://dx.doi.org/10.1038/cddis.2015.184 Text en Copyright © 2015 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Sheng, L He, P Yang, X Zhou, M Feng, Q
spellingShingle	Sheng, L He, P Yang, X Zhou, M Feng, Q miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
author_facet	Sheng, L He, P Yang, X Zhou, M Feng, Q
author_sort	Sheng, L
title	miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
title_short	miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
title_full	miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
title_fullStr	miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
title_full_unstemmed	miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2
title_sort	mir-612 negatively regulates colorectal cancer growth and metastasis by targeting akt2
description	Colorectal cancer (CRC) is one of the most common cancers worldwide, with a particularly high incidence in developed countries. Distant metastasis and recurrence are the main causes of CRC-related deaths. MicroRNAs (miRNAs) in the serum make them potential biomarkers for cancers, as reported in serum or tumor tissues from CRC patients. In this study, we found that miR-612 expression was significantly lower in CRC tissues or cells compared with peritumor tissues or normal cells, and lower in metastatic CRC specimens compared with non-metastatic specimens, whereas AKT2 exhibited opposite trend. Gain-of-function and loss-of-function assays showed that miR-612 inhibited CRC cell proliferation and migration in vitro by Cell Counting Kit-8 and transwell assays. Further analysis revealed that miR-612 directly suppressed AKT2, which in turn inhibited the downstream epithelial–mesenchymal transition-related signaling pathway. These results were additionally validated in vivo by tumorigenesis and liver metastasis experiments. The results of this study suggested a critical role of miR-612 in the development of CRC.
publisher	Nature Publishing Group
publishDate	2015
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4650731/
_version_	1613503209312092160

miR-612 negatively regulates colorectal cancer growth and metastasis by targeting AKT2

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